Study of myoblast differentiation using multi-dimensional scaffolds consisting of nano and micropatterns

Sung Ho Cha, Hyun Jong Lee, Won-Gun Koh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: The topographical cue is major influence on skeletal muscle cell culture because the structure is highly organized and consists of long parallel bundles of multinucleated myotubes that are formed by differentiation and fusion of myoblast satellite cells. In this technical report, we fabricated a multiscale scaffold using electrospinning and poly (ethylene glycol) (PEG) hydrogel micropatterns to monitor the cell behaviors on nano- and micro-alignment combined scaffolds with different combinations of angles. Results: We fabricated multiscale scaffolds that provide biocompatible and extracellular matrix (ECM)-mimetic environments via electrospun nanofiber and PEG hydrogel micro patterning. MTT assays demonstrated an almost four-fold increase in the proliferation rate during the 7 days of cell culture for all of the experimental groups. Cell orientation and elongation were measured to confirm the myogenic potential. On the aligned fibrous scaffolds, more than 90% of the cells were dispersed ± 20° of the fiber orientation. To determine cell elongation, we monitored nuclei aspect ratios. On a random nanofiber, the cells demonstrated an aspect ratio of 1.33, but on perpendicular and parallel nanofibers, the aspect ratio was greater than 2. Myosin heavy chain (MHC) expression was significantly higher i) on parallel compared to random fibers, ii) the 100 μm compared to the 200 μm line pattern. We confirmed the disparate trends of myotube formation that can be provoked through multi-dimensional scaffolds. Conclusion: We studied more favorable environments that induce cell alignment and elongation for myogenesis by combining nano- and micro-scale patterns. The fabricated system can serve as a novel multi-dimensional platform to study in vitro cell behaviors.

Original languageEnglish
Article number1
JournalBiomaterials Research
Volume21
Issue number1
DOIs
Publication statusPublished - 2017 Jan 1

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Myoblasts
Scaffolds
Nanofibers
Polyethylene glycols
Aspect ratio
Elongation
Hydrogel
Cell culture
Hydrogels
Skeletal Muscle Fibers
Myosin Heavy Chains
Electrospinning
Fiber reinforced materials
Cell Culture Techniques
Muscle
Assays
Fusion reactions
Ethylene Glycol
Muscle Development
Satellites

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

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abstract = "Background: The topographical cue is major influence on skeletal muscle cell culture because the structure is highly organized and consists of long parallel bundles of multinucleated myotubes that are formed by differentiation and fusion of myoblast satellite cells. In this technical report, we fabricated a multiscale scaffold using electrospinning and poly (ethylene glycol) (PEG) hydrogel micropatterns to monitor the cell behaviors on nano- and micro-alignment combined scaffolds with different combinations of angles. Results: We fabricated multiscale scaffolds that provide biocompatible and extracellular matrix (ECM)-mimetic environments via electrospun nanofiber and PEG hydrogel micro patterning. MTT assays demonstrated an almost four-fold increase in the proliferation rate during the 7 days of cell culture for all of the experimental groups. Cell orientation and elongation were measured to confirm the myogenic potential. On the aligned fibrous scaffolds, more than 90{\%} of the cells were dispersed ± 20° of the fiber orientation. To determine cell elongation, we monitored nuclei aspect ratios. On a random nanofiber, the cells demonstrated an aspect ratio of 1.33, but on perpendicular and parallel nanofibers, the aspect ratio was greater than 2. Myosin heavy chain (MHC) expression was significantly higher i) on parallel compared to random fibers, ii) the 100 μm compared to the 200 μm line pattern. We confirmed the disparate trends of myotube formation that can be provoked through multi-dimensional scaffolds. Conclusion: We studied more favorable environments that induce cell alignment and elongation for myogenesis by combining nano- and micro-scale patterns. The fabricated system can serve as a novel multi-dimensional platform to study in vitro cell behaviors.",
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Study of myoblast differentiation using multi-dimensional scaffolds consisting of nano and micropatterns. / Cha, Sung Ho; Lee, Hyun Jong; Koh, Won-Gun.

In: Biomaterials Research, Vol. 21, No. 1, 1, 01.01.2017.

Research output: Contribution to journalArticle

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