Thermophilic microorganisms capable of producing chitinase enzymes were screened from samples collected from several crater and geothermal areas. The chitinolytic microorganisms were grown in a selective medium contai containing colloidal chitin. The Bacillus K29-14 isolate was found to exhibit the highest chitinase and chitin deacetylase activities. When grown in a chitin-containing medium, the isolate produced extracellular chitinase after 24 h of incubation. The optimum temperature and pH for the chitinase were 55°C and pH 7, respectively, while those for the chitin deacetylase were 55°C and pH 8, respectively. The thermostable chitinase and chitin deacetylase also retained 80-90% of their activity after incubation for 5 h at 70°C. The divalent cations CoCl2 and NiCl2 increased the chitinase activity, while ZnCl2 inhibited the enzyme. The chitin deacetylase was also activated by the presence of MgCl2 and inhibited by MnCl2, NiCl2, and CaCl2. A zymogram analysis revealed several forms of chitinase, with a 67 kDa form being the major enzyme.
|Number of pages||6|
|Journal||Journal of microbiology and biotechnology|
|Publication status||Published - 2004 Aug 1|
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology