Sub-lethal hyperthermia promotes epithelial-to-mesenchymal-like transition of breast cancer cells

implication of the synergy between hyperthermia and chemotherapy

Tae Hee Lee, Jiyoon Bu, Byoung Hyuck Kim, Michael J. Poellmann, Seungpyo Hong, Sung Hee Hyun

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Thermotherapy has demonstrated a potential to be an effective non-surgical technique to treat breast cancer. Despite its advantages, including low toxicity and high repeatability, thermotherapy is typically required to be applied in combination with other treatments since the residual tumor cells that survive after hyperthermal treatment often cause recurrence. In this study, we confirmed that breast cancer cells tolerate temperature of up to 47 °C by synthesizing a large amount of heat shock proteins. Further changes in the molecular properties of the heat-exposed cells were investigated using western blotting, quantitative reverse transcription polymerase chain reaction, and immunocytochemistry. We found that low-temperature hyperthermia promoted epithelial-to-mesenchymal-like transition (EMT), as observed by the increased mesenchymal marker expression levels while decreasing epithelial markers. Moreover, cell morphology changed from cobblestone-like to a more spindle-like appearance, in addition to significantly enhanced cell motility upon heat treatment. These results all support that sub-lethal hyperthermal stress induces EMT. In addition, we examined changes in the chemo-sensitivity of the heat-treated cells. Addition of a chemo-drugs caused increased cytotoxicity of the heat-treated cells compared to the cells that were not co-treated with heat. Our study demonstrates that thermotherapy alone may cause undesirable EMT, which could be well overcome through a synergistic effect when applied with chemotherapy.

Original languageEnglish
Pages (from-to)52-57
Number of pages6
JournalRSC Advances
Volume9
Issue number1
DOIs
Publication statusPublished - 2019 Jan 1

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Chemotherapy
Cells
Polymerase chain reaction
Transcription
Cytotoxicity
Heat-Shock Proteins
Toxicity
Tumors
Heat treatment
Temperature
Hot Temperature
Proteins
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Lee, Tae Hee ; Bu, Jiyoon ; Kim, Byoung Hyuck ; Poellmann, Michael J. ; Hong, Seungpyo ; Hyun, Sung Hee. / Sub-lethal hyperthermia promotes epithelial-to-mesenchymal-like transition of breast cancer cells : implication of the synergy between hyperthermia and chemotherapy. In: RSC Advances. 2019 ; Vol. 9, No. 1. pp. 52-57.
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Sub-lethal hyperthermia promotes epithelial-to-mesenchymal-like transition of breast cancer cells : implication of the synergy between hyperthermia and chemotherapy. / Lee, Tae Hee; Bu, Jiyoon; Kim, Byoung Hyuck; Poellmann, Michael J.; Hong, Seungpyo; Hyun, Sung Hee.

In: RSC Advances, Vol. 9, No. 1, 01.01.2019, p. 52-57.

Research output: Contribution to journalArticle

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