Sublingual immunization with a live attenuated influenza A virus lacking the nonstructural protein 1 induces broad protective immunity in mice

Hae Jung Park, Boris Ferko, Young Ho Byun, Joo Hye Song, Gye Yeong Han, Elisabeth Roethl, Andrej Egorov, Thomas Muster, Baiklin Seong, Mi Na Kweon, Manki Song, Cecil Czerkinsky, Huan H. Nguyen

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The nonstructural protein 1 (NS1) of influenza A virus (IAV) enables the virus to disarm the host cell type 1 IFN defense system. Mutation or deletion of the NS1 gene leads to attenuation of the virus and enhances host antiviral response making such live-attenuated influenza viruses attractive vaccine candidates. Sublingual (SL) immunization with live influenza virus has been found to be safe and effective for inducing protective immune responses in mucosal and systemic compartments. Here we demonstrate that SL immunization with NS1 deleted IAV (DeltaNS1 H1N1 or DeltaNS1 H5N1) induced protection against challenge with homologous as well as heterosubtypic influenza viruses. Protection was comparable with that induced by intranasal (IN) immunization and was associated with high levels of virus-specific antibodies (Abs). SL immunization with DeltaNS1 virus induced broad Ab responses in mucosal and systemic compartments and stimulated immune cells in mucosa-associated and systemic lymphoid organs. Thus, SL immunization with DeltaNS1 offers a novel potential vaccination strategy for the control of influenza outbreaks including pandemics.

Original languageEnglish
Article numbere39921
JournalPloS one
Volume7
Issue number6
DOIs
Publication statusPublished - 2012 Jun 27

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Immunization
Influenza A virus
Viruses
Immunity
immunization
immunity
Orthomyxoviridae
mice
viruses
Proteins
proteins
mucosal immunity
Mucosal Immunity
Influenza Vaccines
Sequence Deletion
Pandemics
pandemic
influenza
Human Influenza
mucosa

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Park, Hae Jung ; Ferko, Boris ; Byun, Young Ho ; Song, Joo Hye ; Han, Gye Yeong ; Roethl, Elisabeth ; Egorov, Andrej ; Muster, Thomas ; Seong, Baiklin ; Kweon, Mi Na ; Song, Manki ; Czerkinsky, Cecil ; Nguyen, Huan H. / Sublingual immunization with a live attenuated influenza A virus lacking the nonstructural protein 1 induces broad protective immunity in mice. In: PloS one. 2012 ; Vol. 7, No. 6.
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Park, HJ, Ferko, B, Byun, YH, Song, JH, Han, GY, Roethl, E, Egorov, A, Muster, T, Seong, B, Kweon, MN, Song, M, Czerkinsky, C & Nguyen, HH 2012, 'Sublingual immunization with a live attenuated influenza A virus lacking the nonstructural protein 1 induces broad protective immunity in mice', PloS one, vol. 7, no. 6, e39921. https://doi.org/10.1371/journal.pone.0039921

Sublingual immunization with a live attenuated influenza A virus lacking the nonstructural protein 1 induces broad protective immunity in mice. / Park, Hae Jung; Ferko, Boris; Byun, Young Ho; Song, Joo Hye; Han, Gye Yeong; Roethl, Elisabeth; Egorov, Andrej; Muster, Thomas; Seong, Baiklin; Kweon, Mi Na; Song, Manki; Czerkinsky, Cecil; Nguyen, Huan H.

In: PloS one, Vol. 7, No. 6, e39921, 27.06.2012.

Research output: Contribution to journalArticle

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AU - Park, Hae Jung

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AU - Roethl, Elisabeth

AU - Egorov, Andrej

AU - Muster, Thomas

AU - Seong, Baiklin

AU - Kweon, Mi Na

AU - Song, Manki

AU - Czerkinsky, Cecil

AU - Nguyen, Huan H.

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