Subpixel resolving optofluidic microscope based on super resolution algorithm

Guoan Zheng, Seung Ah Lee, Samuel Yang, Changhuei Yang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We report the implementation of a fully on-chip, lensless, sub-pixel resolving optofluidic microscope (SROFM) based on the super resolution algorithm. The device utilizes microfluidic flow to deliver specimens directly across a complementary metal oxide semiconductor (CMOS) sensor to generate a sequence of low-resolution (LR) projection images, where resolution is limited by the sensor's pixel size. This image sequence is then processed with a pixel super-resolution algorithm to reconstruct a single high resolution (HR) image, where features beyond the Nyquist rate of the LR images are resolved. We demonstrate the device's capabilities by imaging red blood cell, microspheres, protist Euglena gracilis, and Entamoeba invadens cysts with sub-cellular resolution. We also demonstrate the capability of SROFM for malaria infected red blood cell diagnostics.

Original languageEnglish
Title of host publication2011 8th IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, ISBI'11
Pages1362-1365
Number of pages4
DOIs
Publication statusPublished - 2011 Nov 2
Event2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11 - Chicago, IL, United States
Duration: 2011 Mar 302011 Apr 2

Publication series

NameProceedings - International Symposium on Biomedical Imaging
ISSN (Print)1945-7928
ISSN (Electronic)1945-8452

Other

Other2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11
CountryUnited States
CityChicago, IL
Period11/3/3011/4/2

Fingerprint

Lab-On-A-Chip Devices
Microscopes
Image resolution
Erythrocytes
Pixels
Euglena gracilis
Entamoeba
Semiconductors
Optical resolving power
Microspheres
Oxides
Malaria
Cysts
Blood
Metals
Cells
Equipment and Supplies
Sensors
Microfluidics
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

Cite this

Zheng, G., Lee, S. A., Yang, S., & Yang, C. (2011). Subpixel resolving optofluidic microscope based on super resolution algorithm. In 2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11 (pp. 1362-1365). [5872653] (Proceedings - International Symposium on Biomedical Imaging). https://doi.org/10.1109/ISBI.2011.5872653
Zheng, Guoan ; Lee, Seung Ah ; Yang, Samuel ; Yang, Changhuei. / Subpixel resolving optofluidic microscope based on super resolution algorithm. 2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11. 2011. pp. 1362-1365 (Proceedings - International Symposium on Biomedical Imaging).
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Zheng, G, Lee, SA, Yang, S & Yang, C 2011, Subpixel resolving optofluidic microscope based on super resolution algorithm. in 2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11., 5872653, Proceedings - International Symposium on Biomedical Imaging, pp. 1362-1365, 2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11, Chicago, IL, United States, 11/3/30. https://doi.org/10.1109/ISBI.2011.5872653

Subpixel resolving optofluidic microscope based on super resolution algorithm. / Zheng, Guoan; Lee, Seung Ah; Yang, Samuel; Yang, Changhuei.

2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11. 2011. p. 1362-1365 5872653 (Proceedings - International Symposium on Biomedical Imaging).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Zheng G, Lee SA, Yang S, Yang C. Subpixel resolving optofluidic microscope based on super resolution algorithm. In 2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11. 2011. p. 1362-1365. 5872653. (Proceedings - International Symposium on Biomedical Imaging). https://doi.org/10.1109/ISBI.2011.5872653