Substrate sources regulate spatial variation of metabolically active methanogens from two contrasting freshwater wetlands

Yongxin Lin, Deyan Liu, Weixin Ding, Hojeong Kang, Chris Freeman, Junji Yuan, Jian Xiang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

There is ample evidence that methane (CH4) emissions from natural wetlands exhibit large spatial variations at a field scale. However, little is known about the metabolically active methanogens mediating these differences. We explored the spatial patterns in active methanogens of summer inundated Calamagrostis angustifolia marsh with low CH4 emissions and permanently inundated Carex lasiocarpa marsh with high CH4 emissions in Sanjiang Plain, China. In C. angustifolia marsh, the addition of 13C-acetate significantly increased the CH4 production rate, and Methanosarcinaceae methanogens were found to participate in the consumption of acetate. In C. lasiocarpa marsh, there was no apparent increase in the CH4 production rate and no methanogen species were labeled with 13C. When 13CO2-H2 was added, however, CH4 production was found to be due to Fen Cluster (Methanomicrobiales) in C. angustifolia marsh and Methanobacterium Cluster B (Methanobacteriaceae) together with Fen Cluster in C. lasiocarpa marsh. These results suggested that CH4 was produced primarily by hydrogenotrophic methanogens using substrates mainly derived from plant litter in C. lasiocarpa marsh and by both hydrogenotrophic and acetoclastic methanogens using substrates mainly derived from root exudate in C. angustifolia marsh. The significantly lower CH4 emissions measured in situ in C. angustifolia marsh was primarily due to a deficiency of substrates compared to C. lasiocarpa marsh. Therefore, we speculate that the substrate source regulates both the type of active methanogens and the CH4 production pathway and consequently contributes to the spatial variations in CH4 productions observed in these freshwater marshes.

Original languageEnglish
Pages (from-to)10779-10791
Number of pages13
JournalApplied Microbiology and Biotechnology
Volume99
Issue number24
DOIs
Publication statusPublished - 2015 Dec 1

Fingerprint

Wetlands
Fresh Water
Methanomicrobiales
Methanosarcinaceae
Methanobacteriaceae
Acetates
Methanobacterium
Methane
Exudates and Transudates
China

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Lin, Yongxin ; Liu, Deyan ; Ding, Weixin ; Kang, Hojeong ; Freeman, Chris ; Yuan, Junji ; Xiang, Jian. / Substrate sources regulate spatial variation of metabolically active methanogens from two contrasting freshwater wetlands. In: Applied Microbiology and Biotechnology. 2015 ; Vol. 99, No. 24. pp. 10779-10791.
@article{d2aa4cf7c8ea4ec48a0ec14d38696bc3,
title = "Substrate sources regulate spatial variation of metabolically active methanogens from two contrasting freshwater wetlands",
abstract = "There is ample evidence that methane (CH4) emissions from natural wetlands exhibit large spatial variations at a field scale. However, little is known about the metabolically active methanogens mediating these differences. We explored the spatial patterns in active methanogens of summer inundated Calamagrostis angustifolia marsh with low CH4 emissions and permanently inundated Carex lasiocarpa marsh with high CH4 emissions in Sanjiang Plain, China. In C. angustifolia marsh, the addition of 13C-acetate significantly increased the CH4 production rate, and Methanosarcinaceae methanogens were found to participate in the consumption of acetate. In C. lasiocarpa marsh, there was no apparent increase in the CH4 production rate and no methanogen species were labeled with 13C. When 13CO2-H2 was added, however, CH4 production was found to be due to Fen Cluster (Methanomicrobiales) in C. angustifolia marsh and Methanobacterium Cluster B (Methanobacteriaceae) together with Fen Cluster in C. lasiocarpa marsh. These results suggested that CH4 was produced primarily by hydrogenotrophic methanogens using substrates mainly derived from plant litter in C. lasiocarpa marsh and by both hydrogenotrophic and acetoclastic methanogens using substrates mainly derived from root exudate in C. angustifolia marsh. The significantly lower CH4 emissions measured in situ in C. angustifolia marsh was primarily due to a deficiency of substrates compared to C. lasiocarpa marsh. Therefore, we speculate that the substrate source regulates both the type of active methanogens and the CH4 production pathway and consequently contributes to the spatial variations in CH4 productions observed in these freshwater marshes.",
author = "Yongxin Lin and Deyan Liu and Weixin Ding and Hojeong Kang and Chris Freeman and Junji Yuan and Jian Xiang",
year = "2015",
month = "12",
day = "1",
doi = "10.1007/s00253-015-6912-7",
language = "English",
volume = "99",
pages = "10779--10791",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "24",

}

Substrate sources regulate spatial variation of metabolically active methanogens from two contrasting freshwater wetlands. / Lin, Yongxin; Liu, Deyan; Ding, Weixin; Kang, Hojeong; Freeman, Chris; Yuan, Junji; Xiang, Jian.

In: Applied Microbiology and Biotechnology, Vol. 99, No. 24, 01.12.2015, p. 10779-10791.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Substrate sources regulate spatial variation of metabolically active methanogens from two contrasting freshwater wetlands

AU - Lin, Yongxin

AU - Liu, Deyan

AU - Ding, Weixin

AU - Kang, Hojeong

AU - Freeman, Chris

AU - Yuan, Junji

AU - Xiang, Jian

PY - 2015/12/1

Y1 - 2015/12/1

N2 - There is ample evidence that methane (CH4) emissions from natural wetlands exhibit large spatial variations at a field scale. However, little is known about the metabolically active methanogens mediating these differences. We explored the spatial patterns in active methanogens of summer inundated Calamagrostis angustifolia marsh with low CH4 emissions and permanently inundated Carex lasiocarpa marsh with high CH4 emissions in Sanjiang Plain, China. In C. angustifolia marsh, the addition of 13C-acetate significantly increased the CH4 production rate, and Methanosarcinaceae methanogens were found to participate in the consumption of acetate. In C. lasiocarpa marsh, there was no apparent increase in the CH4 production rate and no methanogen species were labeled with 13C. When 13CO2-H2 was added, however, CH4 production was found to be due to Fen Cluster (Methanomicrobiales) in C. angustifolia marsh and Methanobacterium Cluster B (Methanobacteriaceae) together with Fen Cluster in C. lasiocarpa marsh. These results suggested that CH4 was produced primarily by hydrogenotrophic methanogens using substrates mainly derived from plant litter in C. lasiocarpa marsh and by both hydrogenotrophic and acetoclastic methanogens using substrates mainly derived from root exudate in C. angustifolia marsh. The significantly lower CH4 emissions measured in situ in C. angustifolia marsh was primarily due to a deficiency of substrates compared to C. lasiocarpa marsh. Therefore, we speculate that the substrate source regulates both the type of active methanogens and the CH4 production pathway and consequently contributes to the spatial variations in CH4 productions observed in these freshwater marshes.

AB - There is ample evidence that methane (CH4) emissions from natural wetlands exhibit large spatial variations at a field scale. However, little is known about the metabolically active methanogens mediating these differences. We explored the spatial patterns in active methanogens of summer inundated Calamagrostis angustifolia marsh with low CH4 emissions and permanently inundated Carex lasiocarpa marsh with high CH4 emissions in Sanjiang Plain, China. In C. angustifolia marsh, the addition of 13C-acetate significantly increased the CH4 production rate, and Methanosarcinaceae methanogens were found to participate in the consumption of acetate. In C. lasiocarpa marsh, there was no apparent increase in the CH4 production rate and no methanogen species were labeled with 13C. When 13CO2-H2 was added, however, CH4 production was found to be due to Fen Cluster (Methanomicrobiales) in C. angustifolia marsh and Methanobacterium Cluster B (Methanobacteriaceae) together with Fen Cluster in C. lasiocarpa marsh. These results suggested that CH4 was produced primarily by hydrogenotrophic methanogens using substrates mainly derived from plant litter in C. lasiocarpa marsh and by both hydrogenotrophic and acetoclastic methanogens using substrates mainly derived from root exudate in C. angustifolia marsh. The significantly lower CH4 emissions measured in situ in C. angustifolia marsh was primarily due to a deficiency of substrates compared to C. lasiocarpa marsh. Therefore, we speculate that the substrate source regulates both the type of active methanogens and the CH4 production pathway and consequently contributes to the spatial variations in CH4 productions observed in these freshwater marshes.

UR - http://www.scopus.com/inward/record.url?scp=84948387889&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84948387889&partnerID=8YFLogxK

U2 - 10.1007/s00253-015-6912-7

DO - 10.1007/s00253-015-6912-7

M3 - Article

C2 - 26286511

AN - SCOPUS:84948387889

VL - 99

SP - 10779

EP - 10791

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 24

ER -