Sulfonated Ni(II)porphyrin improves the detection of Z-DNA in condensed and non-condensed BZB DNA sequences

Andrea E. Holmes, Jung Kyu Choi, Jacob Francis, Alessandro D'Urso, Milan Balaz

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We report a very selective and sensitive spectroscopic detection of Z-DNA embedded in B-DNA in condensed as well as non-condensed DNA using anionic Ni(II) meso-tetrakis(4-sulphonatophenyl)porphyrin, NiTPPS. A combination of micromolar concentrations of Ni(II) and spermine 4 + allowed us to prepare left-handed Z-DNA in short oligonucleotides without DNA condensation. A strong induced circular dichroism (ICD) signal was observed in the visible absorption region when NiTPPS was added to BZ DNA (Z-DNA fragment located at the end of a B-DNA tract with one B/Z DNA junction) and BZB DNA (Z-DNA sequence embedded in B-DNA having two B/Z DNA junctions). Almost no ICD signal was detected when NiTPPS was added to B-DNA. NiTPPS showed different binding modes with condensed and non-condensed Z-DNAs and allowed the distinction between condensed Z-DNA (positive bisignate CD couplet) and non-condensed Z-DNA (negative bisignate CD couplet).

Original languageEnglish
Pages (from-to)18-20
Number of pages3
JournalJournal of Inorganic Biochemistry
Volume110
DOIs
Publication statusPublished - 2012 May 1

Fingerprint

Z-Form DNA
DNA sequences
Porphyrins
B-Form DNA
DNA
Circular Dichroism
Spermine
Dichroism
Oligonucleotides
Condensation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Inorganic Chemistry

Cite this

Holmes, Andrea E. ; Choi, Jung Kyu ; Francis, Jacob ; D'Urso, Alessandro ; Balaz, Milan. / Sulfonated Ni(II)porphyrin improves the detection of Z-DNA in condensed and non-condensed BZB DNA sequences. In: Journal of Inorganic Biochemistry. 2012 ; Vol. 110. pp. 18-20.
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abstract = "We report a very selective and sensitive spectroscopic detection of Z-DNA embedded in B-DNA in condensed as well as non-condensed DNA using anionic Ni(II) meso-tetrakis(4-sulphonatophenyl)porphyrin, NiTPPS. A combination of micromolar concentrations of Ni(II) and spermine 4 + allowed us to prepare left-handed Z-DNA in short oligonucleotides without DNA condensation. A strong induced circular dichroism (ICD) signal was observed in the visible absorption region when NiTPPS was added to BZ DNA (Z-DNA fragment located at the end of a B-DNA tract with one B/Z DNA junction) and BZB DNA (Z-DNA sequence embedded in B-DNA having two B/Z DNA junctions). Almost no ICD signal was detected when NiTPPS was added to B-DNA. NiTPPS showed different binding modes with condensed and non-condensed Z-DNAs and allowed the distinction between condensed Z-DNA (positive bisignate CD couplet) and non-condensed Z-DNA (negative bisignate CD couplet).",
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Sulfonated Ni(II)porphyrin improves the detection of Z-DNA in condensed and non-condensed BZB DNA sequences. / Holmes, Andrea E.; Choi, Jung Kyu; Francis, Jacob; D'Urso, Alessandro; Balaz, Milan.

In: Journal of Inorganic Biochemistry, Vol. 110, 01.05.2012, p. 18-20.

Research output: Contribution to journalArticle

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