SUMOylated IRF-1 shows oncogenic potential by mimicking IRF-2

Sun Mi Park, Myounghee Chae, Bo Kyoung Kim, Taegun Seo, Ik Soon Jang, Jong Soon Choi, Il Chul Kim, Je Ho Lee, Junsoo Park

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Interferon regulatory factor-1 (IRF-1) is an interferon-induced transcriptional activator that suppresses tumors by impeding cell proliferation. Recently, we demonstrated that the level of SUMOylated IRF-1 is elevated in tumor cells, and that SUMOylation of IRF-1 attenuates its tumor-suppressive function. Here we report that SUMOylated IRF-1 mimics IRF-2, an antagonistic repressor, and shows oncogenic potential. To demonstrate the role of SUMOylated IRF-1 in tumorigenesis, we used SUMO-IRF-1 recombinant protein. Stable expression of SUMO-IRF-1 in NIH3T3 cells resulted in focus formation and anchorage-independent growth in soft agar. Inoculation of SUMO-IRF-1-transfected cells into athymic nude mice resulted in tumor formation and infiltration of adipose tissues. Finally, we demonstrated that SUMO-IRF-1 transforms NIH3T3 cells in a dose-dependent manner suggesting that SUMOylated IRF-1 may act as an oncogenic protein in tumor cells.

Original languageEnglish
Pages (from-to)926-930
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume391
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1

Fingerprint

Interferon Regulatory Factor-1
Tumors
Neoplasms
Nude Mice
Cells
neurotensin mimic 2
Sumoylation
Cell proliferation
Recombinant Proteins
Infiltration
Interferons
Agar
Adipose Tissue
Carcinogenesis

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Park, Sun Mi ; Chae, Myounghee ; Kim, Bo Kyoung ; Seo, Taegun ; Jang, Ik Soon ; Choi, Jong Soon ; Kim, Il Chul ; Lee, Je Ho ; Park, Junsoo. / SUMOylated IRF-1 shows oncogenic potential by mimicking IRF-2. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 391, No. 1. pp. 926-930.
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abstract = "Interferon regulatory factor-1 (IRF-1) is an interferon-induced transcriptional activator that suppresses tumors by impeding cell proliferation. Recently, we demonstrated that the level of SUMOylated IRF-1 is elevated in tumor cells, and that SUMOylation of IRF-1 attenuates its tumor-suppressive function. Here we report that SUMOylated IRF-1 mimics IRF-2, an antagonistic repressor, and shows oncogenic potential. To demonstrate the role of SUMOylated IRF-1 in tumorigenesis, we used SUMO-IRF-1 recombinant protein. Stable expression of SUMO-IRF-1 in NIH3T3 cells resulted in focus formation and anchorage-independent growth in soft agar. Inoculation of SUMO-IRF-1-transfected cells into athymic nude mice resulted in tumor formation and infiltration of adipose tissues. Finally, we demonstrated that SUMO-IRF-1 transforms NIH3T3 cells in a dose-dependent manner suggesting that SUMOylated IRF-1 may act as an oncogenic protein in tumor cells.",
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Park, SM, Chae, M, Kim, BK, Seo, T, Jang, IS, Choi, JS, Kim, IC, Lee, JH & Park, J 2010, 'SUMOylated IRF-1 shows oncogenic potential by mimicking IRF-2', Biochemical and Biophysical Research Communications, vol. 391, no. 1, pp. 926-930. https://doi.org/10.1016/j.bbrc.2009.11.166

SUMOylated IRF-1 shows oncogenic potential by mimicking IRF-2. / Park, Sun Mi; Chae, Myounghee; Kim, Bo Kyoung; Seo, Taegun; Jang, Ik Soon; Choi, Jong Soon; Kim, Il Chul; Lee, Je Ho; Park, Junsoo.

In: Biochemical and Biophysical Research Communications, Vol. 391, No. 1, 01.01.2010, p. 926-930.

Research output: Contribution to journalArticle

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AU - Chae, Myounghee

AU - Kim, Bo Kyoung

AU - Seo, Taegun

AU - Jang, Ik Soon

AU - Choi, Jong Soon

AU - Kim, Il Chul

AU - Lee, Je Ho

AU - Park, Junsoo

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