SUMOylated IRF-1 shows oncogenic potential by mimicking IRF-2

Sun Mi Park, Myounghee Chae, Bo Kyoung Kim, Taegun Seo, Ik Soon Jang, Jong Soon Choi, Il Chul Kim, Je Ho Lee, Junsoo Park

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Interferon regulatory factor-1 (IRF-1) is an interferon-induced transcriptional activator that suppresses tumors by impeding cell proliferation. Recently, we demonstrated that the level of SUMOylated IRF-1 is elevated in tumor cells, and that SUMOylation of IRF-1 attenuates its tumor-suppressive function. Here we report that SUMOylated IRF-1 mimics IRF-2, an antagonistic repressor, and shows oncogenic potential. To demonstrate the role of SUMOylated IRF-1 in tumorigenesis, we used SUMO-IRF-1 recombinant protein. Stable expression of SUMO-IRF-1 in NIH3T3 cells resulted in focus formation and anchorage-independent growth in soft agar. Inoculation of SUMO-IRF-1-transfected cells into athymic nude mice resulted in tumor formation and infiltration of adipose tissues. Finally, we demonstrated that SUMO-IRF-1 transforms NIH3T3 cells in a dose-dependent manner suggesting that SUMOylated IRF-1 may act as an oncogenic protein in tumor cells.

Original languageEnglish
Pages (from-to)926-930
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume391
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1

Bibliographical note

Funding Information:
This work was supported by a National Research Foundation of Korea Grant funded by the Korean Government ( 2009-0072949 and 2009-0065887 ) and by the Science Research Center (Molecular Therapy Research Center).

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'SUMOylated IRF-1 shows oncogenic potential by mimicking IRF-2'. Together they form a unique fingerprint.

Cite this