Sumoylation of the novel protein hRIPβ is involved in replication protein A deposition in PML nuclear bodies

Junsoo Park, Taegun Seo, Hakzoo Kim, Joonho Choe

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Replication protein A (RPA) is a single-stranded-DNA-binding protein composed of three subunits with molecular masses of 70, 32, and 14 kDa. The protein is involved in multiple processes of eukaryotic DNA metabolism, including DNA replication, repair, and recombination. In Xenopus, Xenopus RPA-interacting protein et has been identified as a carrier molecule of RPA into the nucleus. In this study, human RPA-interacting protein ct (hRIPα) and five novel splice isoforms (named hRIPα, hRIPβ, hRIPγ, hRIPδ1, hRIPδ2, and hRIPδ3 according to the lengths of their encoding peptides) were cloned. Among hRIP isoforms, hRIPα and hRIPβ were found to be the major splice isoforms and to show different subcellular localizations. While hRIPα localized to the cytoplasm, hRIPβ was found in the PML nuclear body. Modification of hRIPβ by sumoylation was found to be required for localization to the PML nuclear body. The results of the present work demonstrate that hRIPβ transports RPA into the PML nuclear body and releases RPA upon UV irradiation. hRIPβ thus plays an important role in RPA deposition in PML nuclear bodies and thereby supplements RPA for DNA metabolism.

Original languageEnglish
Pages (from-to)8202-8214
Number of pages13
JournalMolecular and Cellular Biology
Volume25
Issue number18
DOIs
Publication statusPublished - 2005 Sep 1

Fingerprint

Replication Protein A
Sumoylation
Proteins
Protein Isoforms
Xenopus Proteins
DNA
DNA-Binding Proteins
Protein Transport
Xenopus
DNA Replication
DNA Repair
Genetic Recombination

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "Replication protein A (RPA) is a single-stranded-DNA-binding protein composed of three subunits with molecular masses of 70, 32, and 14 kDa. The protein is involved in multiple processes of eukaryotic DNA metabolism, including DNA replication, repair, and recombination. In Xenopus, Xenopus RPA-interacting protein et has been identified as a carrier molecule of RPA into the nucleus. In this study, human RPA-interacting protein ct (hRIPα) and five novel splice isoforms (named hRIPα, hRIPβ, hRIPγ, hRIPδ1, hRIPδ2, and hRIPδ3 according to the lengths of their encoding peptides) were cloned. Among hRIP isoforms, hRIPα and hRIPβ were found to be the major splice isoforms and to show different subcellular localizations. While hRIPα localized to the cytoplasm, hRIPβ was found in the PML nuclear body. Modification of hRIPβ by sumoylation was found to be required for localization to the PML nuclear body. The results of the present work demonstrate that hRIPβ transports RPA into the PML nuclear body and releases RPA upon UV irradiation. hRIPβ thus plays an important role in RPA deposition in PML nuclear bodies and thereby supplements RPA for DNA metabolism.",
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Sumoylation of the novel protein hRIPβ is involved in replication protein A deposition in PML nuclear bodies. / Park, Junsoo; Seo, Taegun; Kim, Hakzoo; Choe, Joonho.

In: Molecular and Cellular Biology, Vol. 25, No. 18, 01.09.2005, p. 8202-8214.

Research output: Contribution to journalArticle

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