1H-nuclear magnetic resonance spectroscopy-based metabolic assessment in a rat model of obesity induced by a high-fat diet

So Hyun Kim, Seung Ok Yang, Hee Su Kim, Yujin Kim, Taesun Park, Hyung Kyoon Choi

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Abstract

Obesity, whose prevalence is increasing rapidly worldwide, is recognized as a risk factor for diabetes, cardiovascular disease, liver disease, and renal disease. To investigate metabolic changes in the urine of a rat model of obesity induced by a high-fat diet (HFD), rats were divided into the following four groups based on the diet type and degree of weight gain: normal-diet (ND) low gainers, ND high gainers, HFD low gainers, and HFD high gainers. Biochemical analyses of visceral fat-pad weight, plasma, and liver tissues were performed. The 1H-nuclear magnetic resonance (1H-NMR) spectra of urine were analyzed using multivariate statistical analysis to identify the separation of the groups. It was observed that the metabolic profile of urine obtained by 1H-NMR-spectroscopy-based metabolomic analysis differed between ND low gainers and ND high gainers even though these animals consumed the same normal diet. Several key metabolites in urine, such as betaine, taurine, acetone/acetoacetate, phenylacetylglycine, pyruvate, lactate, and citrate contributed to the classification of these two groups. The metabolic profile of urine also differed between ND low gainers and HFD high gainers, which consumed the different diet and showed a different weight gain. This study has identified features of urine metabolites in various groups and demonstrated the reliability of an NMR-based metabolomics approach to investigate the effects of the diet and the physical constitution on obesity.

Original languageEnglish
Pages (from-to)1117-1124
Number of pages8
JournalAnalytical and Bioanalytical Chemistry
Volume395
Issue number4
DOIs
Publication statusPublished - 2009 Oct 1

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High Fat Diet
Nutrition
Nuclear magnetic resonance spectroscopy
Rats
Magnetic Resonance Spectroscopy
Obesity
Fats
Diet
Urine
Metabolomics
Metabolome
Weight Gain
Metabolites
Liver
Betaine
Intra-Abdominal Fat
Taurine
Constitution and Bylaws
Acetone
Nuclear magnetic resonance

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

Kim, So Hyun ; Yang, Seung Ok ; Kim, Hee Su ; Kim, Yujin ; Park, Taesun ; Choi, Hyung Kyoon. / 1H-nuclear magnetic resonance spectroscopy-based metabolic assessment in a rat model of obesity induced by a high-fat diet. In: Analytical and Bioanalytical Chemistry. 2009 ; Vol. 395, No. 4. pp. 1117-1124.
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1H-nuclear magnetic resonance spectroscopy-based metabolic assessment in a rat model of obesity induced by a high-fat diet. / Kim, So Hyun; Yang, Seung Ok; Kim, Hee Su; Kim, Yujin; Park, Taesun; Choi, Hyung Kyoon.

In: Analytical and Bioanalytical Chemistry, Vol. 395, No. 4, 01.10.2009, p. 1117-1124.

Research output: Contribution to journalArticle

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