TY - JOUR
T1 - Suppression of Arabidopsis AtPUB30 resulted in increased tolerance to salt stress during germination
AU - Hwang, Jae Hwan
AU - Seo, Dong Hye
AU - Kang, Bin Goo
AU - Kwak, June M.
AU - Kim, Woo Taek
N1 - Publisher Copyright:
© 2014, Springer-Verlag Berlin Heidelberg.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2014
Y1 - 2014
N2 - Abstract: Based on the in silico expression data, the U-box protein 30 (AtPUB30) from Arabidopsis thaliana was identified as a gene that responds to salt stress. The deduced AtPUB30 protein consists of 448 amino acids with a single U-box motif and five ARM-repeat domains. An in vitro self-ubiquitination assay demonstrated that bacterially expressed AtPUB30 exhibited E3 ubiquitin (Ub) ligase activity and that the U-box domain was essential for the activity. Real-time qRT-PCR and promoter-GUS analyses showed that AtPUB30 was induced by high salinity, but not by drought, cold, or abscisic acid (ABA), in roots but not in shoots. These results suggest that AtPUB30 is an Arabidopsis U-box E3 Ub ligase, the expression of which is selectively enhanced by salt stress in roots. T-DNA-inserted loss-of-function atpub30 mutant plants (atpub30-1 and atpub30-2) were more tolerant to salt stress in the germination stage, as identified by radicle emergence, cotyledon opening, and more vigorous early root growth relative to wild-type plants. Thus, it is likely that AtPUB30 plays a negative role in high salinity tolerance in the germination process. Wild type and mutant plants displayed very similar germination rates when treated with ABA, suggesting that the action of AtPUB30 in the germination stage is ABA independent. The post-germination growth of NaCl-stressed wild type and mutant plants were indistinguishable. Overall, our data suggest that the Arabidopsis U-box E3 Ub ligase AtPUB30 participates in the salt stress tolerance as a negative factor in the germination stage in root tissues.Key message: TheArabidopsisU-box E3 Ub ligase AtPUB30 participates in the salt stress tolerance as a negative factor in an ABA-independent manner during germination.
AB - Abstract: Based on the in silico expression data, the U-box protein 30 (AtPUB30) from Arabidopsis thaliana was identified as a gene that responds to salt stress. The deduced AtPUB30 protein consists of 448 amino acids with a single U-box motif and five ARM-repeat domains. An in vitro self-ubiquitination assay demonstrated that bacterially expressed AtPUB30 exhibited E3 ubiquitin (Ub) ligase activity and that the U-box domain was essential for the activity. Real-time qRT-PCR and promoter-GUS analyses showed that AtPUB30 was induced by high salinity, but not by drought, cold, or abscisic acid (ABA), in roots but not in shoots. These results suggest that AtPUB30 is an Arabidopsis U-box E3 Ub ligase, the expression of which is selectively enhanced by salt stress in roots. T-DNA-inserted loss-of-function atpub30 mutant plants (atpub30-1 and atpub30-2) were more tolerant to salt stress in the germination stage, as identified by radicle emergence, cotyledon opening, and more vigorous early root growth relative to wild-type plants. Thus, it is likely that AtPUB30 plays a negative role in high salinity tolerance in the germination process. Wild type and mutant plants displayed very similar germination rates when treated with ABA, suggesting that the action of AtPUB30 in the germination stage is ABA independent. The post-germination growth of NaCl-stressed wild type and mutant plants were indistinguishable. Overall, our data suggest that the Arabidopsis U-box E3 Ub ligase AtPUB30 participates in the salt stress tolerance as a negative factor in the germination stage in root tissues.Key message: TheArabidopsisU-box E3 Ub ligase AtPUB30 participates in the salt stress tolerance as a negative factor in an ABA-independent manner during germination.
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U2 - 10.1007/s00299-014-1706-4
DO - 10.1007/s00299-014-1706-4
M3 - Article
C2 - 25410251
AN - SCOPUS:84925541491
VL - 34
SP - 277
EP - 289
JO - Plant Cell Reports
JF - Plant Cell Reports
SN - 0721-7714
IS - 2
ER -