Suppression of NF-κB activation and cytokine production by N-acetylcysteine in pancreatic acinar cells

Hyeyoung Kim, Jeong Yeon Seo, Kwan Ho Roh, Joo Weon Lim, Kyung Hwan Kim

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Reactive oxygen species (ROS), generated by infiltrating neutrophils, are considered as an important regulator in the pathogenesis and development of pancreatitis. A hallmark of the inflammatory response is the induction of cytokine gene expression, which may be regulated by oxidant-sensitive transcription factor, nuclear factor-κB (NF-κB). Present study aims to investigate whether neutrophils primed by 4β-phorbol 12β-myristate 13α-acetate (PMA) affect the productions of H2O2 and lipid peroxide (LPO), NF-κB activation and cytokine production in pancreatic acinar cells, and whether these alterations were inhibited by N-acetylcysteine (NAC) and superoxide dismutase (SOD). Neutrophils generated ROS by stimulation with PMA, which was inhibited by NAC and SOD. In acinar cells, PMA-primed neutrophils increased the productions of H2O2, LPO, and cytokines both time and dose dependently. PMA-primed neutrophils resulted in the activation of two species of NF-κB dimers (a p50/p65 heterodimer and a p50 homodimer) in acinar cells. Both NAC and SOD inhibited neutrophil-induced, oxidant-mediated alterations in acinar cells. In conclusion, ROS, generated by neutrophils, activates NF-κB, resulting in upregulation of inflammatory cytokines in acinar cells. Antioxidants such as NAC might be useful antiinflammatory agents by inhibiting oxidant-mediated activation of NF-κB and decreasing cytokine production. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)674-683
Number of pages10
JournalFree Radical Biology and Medicine
Volume29
Issue number7
DOIs
Publication statusPublished - 2000 Oct 16

Fingerprint

Acinar Cells
Acetylcysteine
Neutrophils
Chemical activation
Cytokines
Acetates
Oxidants
Superoxide Dismutase
Reactive Oxygen Species
Lipid Peroxides
Gene expression
Dimers
Anti-Inflammatory Agents
Transcription Factors
Antioxidants
Pancreatitis
phorbol-12-myristate
Up-Regulation
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology (medical)

Cite this

Kim, Hyeyoung ; Seo, Jeong Yeon ; Roh, Kwan Ho ; Lim, Joo Weon ; Kim, Kyung Hwan. / Suppression of NF-κB activation and cytokine production by N-acetylcysteine in pancreatic acinar cells. In: Free Radical Biology and Medicine. 2000 ; Vol. 29, No. 7. pp. 674-683.
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Suppression of NF-κB activation and cytokine production by N-acetylcysteine in pancreatic acinar cells. / Kim, Hyeyoung; Seo, Jeong Yeon; Roh, Kwan Ho; Lim, Joo Weon; Kim, Kyung Hwan.

In: Free Radical Biology and Medicine, Vol. 29, No. 7, 16.10.2000, p. 674-683.

Research output: Contribution to journalArticle

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