Suppression of OsKu80 results in defects in developmental growth and increased telomere length in rice (Oryza sativa L.)

Mi Young Byun, Li Hua Cui, Woo Taek Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The Ku70-Ku80 heterodimer plays a critical role in the maintenance of genomic stability in humans and yeasts. In this report, we identified and characterized OsKu80 in rice, a model monocot crop. OsKu80 forms a heterodimer with OsKu70 in yeast and plant cells, as demonstrated by yeast two-hybrid, in vivo co-immunoprecipitation, and bimolecular fluorescence complementation assays. RNAi-mediated knock-down T3 transgenic rice plants (Ubi:RNAi-OsKu80) displayed a retarded growth phenotype at the post-germination stage. In addition, the Ubi:RNAi-OsKu80 knock-down progeny exhibited noticeably increased telomere length as compared to wild-type rice. These results are discussed with the idea that OsKu80 plays a role in developmental growth and telomere length regulation in rice plants.

Original languageEnglish
Pages (from-to)857-862
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume468
Issue number4
DOIs
Publication statusPublished - 2015 Dec 25

Bibliographical note

Funding Information:
This work was supported by a grant from the Woo Jang Chun Special Project (PJ009106) funded by the Rural Development Administration, Republic of Korea , to W.T.K.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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