Suppression of OsKu80 results in defects in developmental growth and increased telomere length in rice (Oryza sativa L.)

Mi Young Byun, Li Hua Cui, Woo Taek Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The Ku70-Ku80 heterodimer plays a critical role in the maintenance of genomic stability in humans and yeasts. In this report, we identified and characterized OsKu80 in rice, a model monocot crop. OsKu80 forms a heterodimer with OsKu70 in yeast and plant cells, as demonstrated by yeast two-hybrid, in vivo co-immunoprecipitation, and bimolecular fluorescence complementation assays. RNAi-mediated knock-down T3 transgenic rice plants (Ubi:RNAi-OsKu80) displayed a retarded growth phenotype at the post-germination stage. In addition, the Ubi:RNAi-OsKu80 knock-down progeny exhibited noticeably increased telomere length as compared to wild-type rice. These results are discussed with the idea that OsKu80 plays a role in developmental growth and telomere length regulation in rice plants.

Original languageEnglish
Pages (from-to)857-862
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume468
Issue number4
DOIs
Publication statusPublished - 2015 Dec 25

Fingerprint

Telomere
Yeast
RNA Interference
Defects
Yeasts
Growth
Crops
Genomic Instability
Genetically Modified Plants
Assays
Plant Cells
Germination
Fluorescence
Immunoprecipitation
Maintenance
Phenotype
Oryza

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "The Ku70-Ku80 heterodimer plays a critical role in the maintenance of genomic stability in humans and yeasts. In this report, we identified and characterized OsKu80 in rice, a model monocot crop. OsKu80 forms a heterodimer with OsKu70 in yeast and plant cells, as demonstrated by yeast two-hybrid, in vivo co-immunoprecipitation, and bimolecular fluorescence complementation assays. RNAi-mediated knock-down T3 transgenic rice plants (Ubi:RNAi-OsKu80) displayed a retarded growth phenotype at the post-germination stage. In addition, the Ubi:RNAi-OsKu80 knock-down progeny exhibited noticeably increased telomere length as compared to wild-type rice. These results are discussed with the idea that OsKu80 plays a role in developmental growth and telomere length regulation in rice plants.",
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Suppression of OsKu80 results in defects in developmental growth and increased telomere length in rice (Oryza sativa L.). / Byun, Mi Young; Cui, Li Hua; Kim, Woo Taek.

In: Biochemical and Biophysical Research Communications, Vol. 468, No. 4, 25.12.2015, p. 857-862.

Research output: Contribution to journalArticle

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