Suppression of RICE TELOMERE BINDING PROTEIN1 results in severe and gradual developmental defects accompanied by genome instability in rice

Jong Pil Hong, Young Byun Mi, Dal Hoe Koo, Kyungsook An, Jae Wook Bang, Kwon Chung In, Gynheung An, Taek Kim Woo

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Although several potential telomere binding proteins have been identified in higher plants, their in vivo functions are still unknown at the plant level. Both knockout and antisense mutants of RICE TELOMERE BINDING PROTEIN1 (RTBP1) exhibited markedly longer telomeres relative to those of the wild type, indicating that the amount of functional RTBP1 is inversely correlated with telomere length. rtbp1 plants displayed progressive and severe developmental abnormalities in both germination and postgermination growth of vegetative organs over four generations (G1 to G4). Reproductive organ formation, including panicles, stamens, and spikelets, was also gradually and severely impaired in G1 to G4 mutants. Up to 11.4, 17.2, and 26.7% of anaphases in G2, G3, and G4 mutant pollen mother cells, respectively, exhibited one or more chromosomal fusions, and this progressively increasing aberrant morphology was correlated with an increased frequency of anaphase bridges containing telomeric repeat DNA. Furthermore, 35S:anti-RTBP1 plants expressing lower levels of RTBP1 mRNA exhibited developmental phenotypes intermediate between the wild type and mutants in all aspects examined, including telomere length, vegetative and reproductive growth, and degree of genomic anomaly. These results suggest that RTBP1 plays dual roles in rice (Oryza sativa), as both a negative regulator of telomere length and one of positive and functional components for proper architecture of telomeres.

Original languageEnglish
Pages (from-to)1770-1781
Number of pages12
JournalPlant Cell
Volume19
Issue number6
DOIs
Publication statusPublished - 2007 Jun

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Suppression of RICE TELOMERE BINDING PROTEIN1 results in severe and gradual developmental defects accompanied by genome instability in rice'. Together they form a unique fingerprint.

Cite this