Suppression of the ER-localized AAA ATPase NgCDC48 inhibits tobacco growth and development

Hansol Bae, Soo Min Choi, Seong Wook Yang, Hyun Sook Pai, Woo Taek Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

CDC48 is a member of the AAA ATPase superfamily. Yeast CDC48 and its mammalian homolog p97 are implicated in diverse cellular processes, including mitosis, membrane fusion, and ubiquitin-dependent protein degradation. However, the cellular functions of plant CDC48 proteins are largely unknown. In the present study, we performed virus-induced gene silencing (VIGS) screening and found that silencing of a gene encoding a tobacco CDC48 homolog, NgCDC48, resulted in severe abnormalities in leaf and shoot development in tobacco. Furthermore, transgenic tobacco plants (35S:anti-NgCDC48), in which the NgCDC48 gene was suppressed using the antisense RNA method, exhibited severely aberrant development of both vegetative and reproductive organs, resulting in arrested shoot and leaf growth and sterile flowers. Approximately 57-83% of 35S:anti-NgCDC48 plants failed to develop mature organs and died at early stage of development. Scanning electron microscopy showed that both adaxial and abaxial epidermal pavement cells in antisense transgenic leaves were significantly smaller and more numerous than those in wild type leaves. These results indicate that NgCDC48 is critically involved in cell growth and development of tobacco plants. An in vivo targeting experiment revealed that NgCDC48 resides in the endoplasmic reticulum (ER) in tobacco protoplasts. We consider the tantalizing possibility that CDC48-mediated degradation of an as-yet unidentified protein(s) in the ER might be a critical step for cell growth and expansion in tobacco leaves.

Original languageEnglish
Pages (from-to)57-65
Number of pages9
JournalMolecules and cells
Volume28
Issue number1
DOIs
Publication statusPublished - 2009 Sep 10

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Growth and Development
Endoplasmic Reticulum
Tobacco
Adenosine Triphosphatases
Gene Silencing
Antisense RNA
Plant Proteins
Membrane Fusion
Protoplasts
Genetically Modified Plants
Growth
Ubiquitin
Mitosis
Electron Scanning Microscopy
Proteolysis
Yeasts
Viruses
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

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title = "Suppression of the ER-localized AAA ATPase NgCDC48 inhibits tobacco growth and development",
abstract = "CDC48 is a member of the AAA ATPase superfamily. Yeast CDC48 and its mammalian homolog p97 are implicated in diverse cellular processes, including mitosis, membrane fusion, and ubiquitin-dependent protein degradation. However, the cellular functions of plant CDC48 proteins are largely unknown. In the present study, we performed virus-induced gene silencing (VIGS) screening and found that silencing of a gene encoding a tobacco CDC48 homolog, NgCDC48, resulted in severe abnormalities in leaf and shoot development in tobacco. Furthermore, transgenic tobacco plants (35S:anti-NgCDC48), in which the NgCDC48 gene was suppressed using the antisense RNA method, exhibited severely aberrant development of both vegetative and reproductive organs, resulting in arrested shoot and leaf growth and sterile flowers. Approximately 57-83{\%} of 35S:anti-NgCDC48 plants failed to develop mature organs and died at early stage of development. Scanning electron microscopy showed that both adaxial and abaxial epidermal pavement cells in antisense transgenic leaves were significantly smaller and more numerous than those in wild type leaves. These results indicate that NgCDC48 is critically involved in cell growth and development of tobacco plants. An in vivo targeting experiment revealed that NgCDC48 resides in the endoplasmic reticulum (ER) in tobacco protoplasts. We consider the tantalizing possibility that CDC48-mediated degradation of an as-yet unidentified protein(s) in the ER might be a critical step for cell growth and expansion in tobacco leaves.",
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Suppression of the ER-localized AAA ATPase NgCDC48 inhibits tobacco growth and development. / Bae, Hansol; Choi, Soo Min; Yang, Seong Wook; Pai, Hyun Sook; Kim, Woo Taek.

In: Molecules and cells, Vol. 28, No. 1, 10.09.2009, p. 57-65.

Research output: Contribution to journalArticle

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