Suppressive mechanism of salmosin, a novel disintegrin in B16 melanoma cell metastasis

In Cheol Kang, Doo Sik Kim, Yangsoo Jang, Kwang Hoe Chung

Research output: Contribution to journalArticle

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Abstract

We have previously reported that salmosin, a novel disintegrin, was isolated from Korean snake (Agkistrodon halys brevicaudus) venom and significantly inhibited solid tumor growth in mice by perturbation of tumor-specific angiogenesis via blocking αvβ3 integrin expressed on vascular endothelial cells. In this study, we investigated the functional specificity of salmosin in tumor cell metastasis. Recombinant salmosin expressed in E. coli that has the RGD sequence markedly inhibited both B16F10 melanoma cell adhesion to the extracellular matrix proteins as well as B16F10 melanoma cell invasion through Matrigel-coated filter. The inhibition by salmosin can be caused by blocking integrins expressed on the surface of B16F10 melanoma cells. Salmosin significantly inhibited the proliferation of B16F10 melanoma cells on the plate coated with collagen I in a dose-dependent manner. In vivo B16F10 melanoma experimental metastasis, salmosin showed remarkable significant inhibitory effect on lung tumor colonization in a concentration-dependent manner. These results clearly demonstrate that antimetastatic activity of salmosin resulted from blocking the integrin-mediated adherence and αvβ3 integrin-mediated proliferation of the melanoma cells. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)169-173
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume275
Issue number1
DOIs
Publication statusPublished - 2000 Aug 18

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Disintegrins
Experimental Melanomas
Neoplasm Metastasis
Melanoma
Integrins
Tumors
Neoplasms
Agkistrodon
Snakes
Extracellular Matrix Proteins
Cell adhesion
Endothelial cells
Venoms
salmosin
Cell Adhesion
Escherichia coli
Collagen
Endothelial Cells
Cells
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "We have previously reported that salmosin, a novel disintegrin, was isolated from Korean snake (Agkistrodon halys brevicaudus) venom and significantly inhibited solid tumor growth in mice by perturbation of tumor-specific angiogenesis via blocking αvβ3 integrin expressed on vascular endothelial cells. In this study, we investigated the functional specificity of salmosin in tumor cell metastasis. Recombinant salmosin expressed in E. coli that has the RGD sequence markedly inhibited both B16F10 melanoma cell adhesion to the extracellular matrix proteins as well as B16F10 melanoma cell invasion through Matrigel-coated filter. The inhibition by salmosin can be caused by blocking integrins expressed on the surface of B16F10 melanoma cells. Salmosin significantly inhibited the proliferation of B16F10 melanoma cells on the plate coated with collagen I in a dose-dependent manner. In vivo B16F10 melanoma experimental metastasis, salmosin showed remarkable significant inhibitory effect on lung tumor colonization in a concentration-dependent manner. These results clearly demonstrate that antimetastatic activity of salmosin resulted from blocking the integrin-mediated adherence and αvβ3 integrin-mediated proliferation of the melanoma cells. (C) 2000 Academic Press.",
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Suppressive mechanism of salmosin, a novel disintegrin in B16 melanoma cell metastasis. / Kang, In Cheol; Kim, Doo Sik; Jang, Yangsoo; Chung, Kwang Hoe.

In: Biochemical and Biophysical Research Communications, Vol. 275, No. 1, 18.08.2000, p. 169-173.

Research output: Contribution to journalArticle

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