Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

Hyunjung Cha, Hyeran Kim, Younju Joung, Hyunju Kang, Jeong Moon, Hyowon Jang, Sohyun Park, Hyung Jun Kwon, In Chul Lee, Sunjoo Kim, Dongeun Yong, Sun Woo Yoon, Sung Gyu Park, Kyeonghye Guk, Eun Kyung Lim, Hyun Gyu Park, Jaebum Choo, Juyeon Jung, Taejoon Kang

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed for the detection of SARS-CoV-2, advanced sensing methods are required for the diagnosis, screening, and surveillance of coronavirus disease 2019 (COVID-19). Here, we report a surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2. The selected antibody pair against the SARS-CoV-2 antigen, along with the magnetic beads, facilitates the accurate direct detection of the virus. The hollow Au NPs exhibit strong, reproducible SERS signals, allowing sensitive quantitative detection of SARS-CoV-2. This assay had detection limits of 2.56 fg/mL for the SARS-CoV-2 antigen and 3.4 plaque-forming units/mL for the SARS-CoV-2 lysates. Furthermore, it facilitated the identification of SARS-CoV-2 in human nasopharyngeal aspirates and diagnosis of COVID-19 within 30 min using a portable Raman device. Thus, this assay can be potentially used for the diagnosis and prevention of COVID-19.

Original languageEnglish
Article number114008
JournalBiosensors and Bioelectronics
Publication statusPublished - 2022 Apr 15

Bibliographical note

Funding Information:
This work was supported by the National R&D Programs through the National Research Foundation (NRF) of Korea funded by the Ministry of Science and ICT (MSIT) of Korea [ NRF-2021M3E5E3080379 , NRF-2021M3E5E3080382 , NRF-2021M3H4A1A02051048 , NRF-2018M3A9E2022821 , NRF-2019R1A2C3004375 , NRF-2020R1A5A1018052 , NRF-2020R1A2C1010453 , and NRF-2021M3E5E3080844 ]; the Global Frontier Program through the Center for BioNano Health Guard funded by the MSIT of Korea [ H-GUARD_2013M3A6B2078950 and H-GUARD_2014M3A6B2060507 ]; the National Research Council of Science & Technology (NST) grant by the MSIT of Korea [ CRC21021-100 ]; the Technology Development Program for Biological Hazards Management in Indoor Air through the Korea Environment Industry & Technology Institute (KEITI) funded by the Ministry of Environment (ME) of Korea [ 2021003370003 ]; the Industrial Technology Alchemist Program funded by Ministry of Trade, Industry, and Energy ( MOTIE ) of Korea [ 20012435 ]; the Nanomedical Devices Development Program of the National Nano Fab Center [ CSM2105M101 ]; and the KRIBB Research Initiative Program [ 1711134081 ]. The funding source played no role in study design; in the collection, analysis, and interpretation of data; in the writing of the report; or the decision to submit the article for publication.

Publisher Copyright:
© 2022 The Authors

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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