Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed for the detection of SARS-CoV-2, advanced sensing methods are required for the diagnosis, screening, and surveillance of coronavirus disease 2019 (COVID-19). Here, we report a surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2. The selected antibody pair against the SARS-CoV-2 antigen, along with the magnetic beads, facilitates the accurate direct detection of the virus. The hollow Au NPs exhibit strong, reproducible SERS signals, allowing sensitive quantitative detection of SARS-CoV-2. This assay had detection limits of 2.56 fg/mL for the SARS-CoV-2 antigen and 3.4 plaque-forming units/mL for the SARS-CoV-2 lysates. Furthermore, it facilitated the identification of SARS-CoV-2 in human nasopharyngeal aspirates and diagnosis of COVID-19 within 30 min using a portable Raman device. Thus, this assay can be potentially used for the diagnosis and prevention of COVID-19.
Original language | English |
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Article number | 114008 |
Journal | Biosensors and Bioelectronics |
Volume | 202 |
DOIs | |
Publication status | Published - 2022 Apr 15 |
Bibliographical note
Funding Information:This work was supported by the National R&D Programs through the National Research Foundation (NRF) of Korea funded by the Ministry of Science and ICT (MSIT) of Korea [ NRF-2021M3E5E3080379 , NRF-2021M3E5E3080382 , NRF-2021M3H4A1A02051048 , NRF-2018M3A9E2022821 , NRF-2019R1A2C3004375 , NRF-2020R1A5A1018052 , NRF-2020R1A2C1010453 , and NRF-2021M3E5E3080844 ]; the Global Frontier Program through the Center for BioNano Health Guard funded by the MSIT of Korea [ H-GUARD_2013M3A6B2078950 and H-GUARD_2014M3A6B2060507 ]; the National Research Council of Science & Technology (NST) grant by the MSIT of Korea [ CRC21021-100 ]; the Technology Development Program for Biological Hazards Management in Indoor Air through the Korea Environment Industry & Technology Institute (KEITI) funded by the Ministry of Environment (ME) of Korea [ 2021003370003 ]; the Industrial Technology Alchemist Program funded by Ministry of Trade, Industry, and Energy ( MOTIE ) of Korea [ 20012435 ]; the Nanomedical Devices Development Program of the National Nano Fab Center [ CSM2105M101 ]; and the KRIBB Research Initiative Program [ 1711134081 ]. The funding source played no role in study design; in the collection, analysis, and interpretation of data; in the writing of the report; or the decision to submit the article for publication.
Publisher Copyright:
© 2022 The Authors
All Science Journal Classification (ASJC) codes
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry