Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

Hyunjung Cha; Hyeran Kim; Younju Joung; Hyunju Kang; Jeong Moon; Hyowon Jang; Sohyun Park; Hyung Jun Kwon; In Chul Lee; Sunjoo Kim; Dongeun Yong; Sun Woo Yoon; Sung Gyu Park; Kyeonghye Guk; Eun Kyung Lim; Hyun Gyu Park; Jaebum Choo; Juyeon Jung; Taejoon Kang

doi:10.1016/j.bios.2022.114008

Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

Hyunjung Cha, Hyeran Kim, Younju Joung, Hyunju Kang, Jeong Moon, Hyowon Jang, Sohyun Park, Hyung Jun Kwon, In Chul Lee, Sunjoo Kim, Dongeun Yong, Sun Woo Yoon, Sung Gyu Park, Kyeonghye Guk, Eun Kyung Lim, Hyun Gyu Park, Jaebum Choo, Juyeon Jung, Taejoon Kang

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed for the detection of SARS-CoV-2, advanced sensing methods are required for the diagnosis, screening, and surveillance of coronavirus disease 2019 (COVID-19). Here, we report a surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2. The selected antibody pair against the SARS-CoV-2 antigen, along with the magnetic beads, facilitates the accurate direct detection of the virus. The hollow Au NPs exhibit strong, reproducible SERS signals, allowing sensitive quantitative detection of SARS-CoV-2. This assay had detection limits of 2.56 fg/mL for the SARS-CoV-2 antigen and 3.4 plaque-forming units/mL for the SARS-CoV-2 lysates. Furthermore, it facilitated the identification of SARS-CoV-2 in human nasopharyngeal aspirates and diagnosis of COVID-19 within 30 min using a portable Raman device. Thus, this assay can be potentially used for the diagnosis and prevention of COVID-19.

Original language	English
Article number	114008
Journal	Biosensors and Bioelectronics
Volume	202
DOIs	https://doi.org/10.1016/j.bios.2022.114008
Publication status	Published - 2022 Apr 15

Bibliographical note

Funding Information:
This work was supported by the National R&D Programs through the National Research Foundation (NRF) of Korea funded by the Ministry of Science and ICT (MSIT) of Korea [ NRF-2021M3E5E3080379 , NRF-2021M3E5E3080382 , NRF-2021M3H4A1A02051048 , NRF-2018M3A9E2022821 , NRF-2019R1A2C3004375 , NRF-2020R1A5A1018052 , NRF-2020R1A2C1010453 , and NRF-2021M3E5E3080844 ]; the Global Frontier Program through the Center for BioNano Health Guard funded by the MSIT of Korea [ H-GUARD_2013M3A6B2078950 and H-GUARD_2014M3A6B2060507 ]; the National Research Council of Science & Technology (NST) grant by the MSIT of Korea [ CRC21021-100 ]; the Technology Development Program for Biological Hazards Management in Indoor Air through the Korea Environment Industry & Technology Institute (KEITI) funded by the Ministry of Environment (ME) of Korea [ 2021003370003 ]; the Industrial Technology Alchemist Program funded by Ministry of Trade, Industry, and Energy ( MOTIE ) of Korea [ 20012435 ]; the Nanomedical Devices Development Program of the National Nano Fab Center [ CSM2105M101 ]; and the KRIBB Research Initiative Program [ 1711134081 ]. The funding source played no role in study design; in the collection, analysis, and interpretation of data; in the writing of the report; or the decision to submit the article for publication.

Publisher Copyright:
© 2022 The Authors

All Science Journal Classification (ASJC) codes

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bios.2022.114008

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Cha, H., Kim, H., Joung, Y., Kang, H., Moon, J., Jang, H., Park, S., Kwon, H. J., Lee, I. C., Kim, S., Yong, D., Yoon, S. W., Park, S. G., Guk, K., Lim, E. K., Park, H. G., Choo, J., Jung, J., & Kang, T. (2022). Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2. Biosensors and Bioelectronics, 202, [114008]. https://doi.org/10.1016/j.bios.2022.114008

Cha, Hyunjung ; Kim, Hyeran ; Joung, Younju ; Kang, Hyunju ; Moon, Jeong ; Jang, Hyowon ; Park, Sohyun ; Kwon, Hyung Jun ; Lee, In Chul ; Kim, Sunjoo ; Yong, Dongeun ; Yoon, Sun Woo ; Park, Sung Gyu ; Guk, Kyeonghye ; Lim, Eun Kyung ; Park, Hyun Gyu ; Choo, Jaebum ; Jung, Juyeon ; Kang, Taejoon. / Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2. In: Biosensors and Bioelectronics. 2022 ; Vol. 202.

@article{059a556db30a4f41a6af4c35e4219aad,

title = "Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2",

abstract = "Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed for the detection of SARS-CoV-2, advanced sensing methods are required for the diagnosis, screening, and surveillance of coronavirus disease 2019 (COVID-19). Here, we report a surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2. The selected antibody pair against the SARS-CoV-2 antigen, along with the magnetic beads, facilitates the accurate direct detection of the virus. The hollow Au NPs exhibit strong, reproducible SERS signals, allowing sensitive quantitative detection of SARS-CoV-2. This assay had detection limits of 2.56 fg/mL for the SARS-CoV-2 antigen and 3.4 plaque-forming units/mL for the SARS-CoV-2 lysates. Furthermore, it facilitated the identification of SARS-CoV-2 in human nasopharyngeal aspirates and diagnosis of COVID-19 within 30 min using a portable Raman device. Thus, this assay can be potentially used for the diagnosis and prevention of COVID-19.",

author = "Hyunjung Cha and Hyeran Kim and Younju Joung and Hyunju Kang and Jeong Moon and Hyowon Jang and Sohyun Park and Kwon, {Hyung Jun} and Lee, {In Chul} and Sunjoo Kim and Dongeun Yong and Yoon, {Sun Woo} and Park, {Sung Gyu} and Kyeonghye Guk and Lim, {Eun Kyung} and Park, {Hyun Gyu} and Jaebum Choo and Juyeon Jung and Taejoon Kang",

note = "Funding Information: This work was supported by the National R&D Programs through the National Research Foundation (NRF) of Korea funded by the Ministry of Science and ICT (MSIT) of Korea [ NRF-2021M3E5E3080379 , NRF-2021M3E5E3080382 , NRF-2021M3H4A1A02051048 , NRF-2018M3A9E2022821 , NRF-2019R1A2C3004375 , NRF-2020R1A5A1018052 , NRF-2020R1A2C1010453 , and NRF-2021M3E5E3080844 ]; the Global Frontier Program through the Center for BioNano Health Guard funded by the MSIT of Korea [ H-GUARD_2013M3A6B2078950 and H-GUARD_2014M3A6B2060507 ]; the National Research Council of Science & Technology (NST) grant by the MSIT of Korea [ CRC21021-100 ]; the Technology Development Program for Biological Hazards Management in Indoor Air through the Korea Environment Industry & Technology Institute (KEITI) funded by the Ministry of Environment (ME) of Korea [ 2021003370003 ]; the Industrial Technology Alchemist Program funded by Ministry of Trade, Industry, and Energy ( MOTIE ) of Korea [ 20012435 ]; the Nanomedical Devices Development Program of the National Nano Fab Center [ CSM2105M101 ]; and the KRIBB Research Initiative Program [ 1711134081 ]. The funding source played no role in study design; in the collection, analysis, and interpretation of data; in the writing of the report; or the decision to submit the article for publication. Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

month = apr,

day = "15",

doi = "10.1016/j.bios.2022.114008",

language = "English",

volume = "202",

journal = "Biosensors and Bioelectronics",

issn = "0956-5663",

publisher = "Elsevier Limited",

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Cha, H, Kim, H, Joung, Y, Kang, H, Moon, J, Jang, H, Park, S, Kwon, HJ, Lee, IC, Kim, S, Yong, D, Yoon, SW, Park, SG, Guk, K, Lim, EK, Park, HG, Choo, J, Jung, J & Kang, T 2022, 'Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2', Biosensors and Bioelectronics, vol. 202, 114008. https://doi.org/10.1016/j.bios.2022.114008

Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2. / Cha, Hyunjung; Kim, Hyeran; Joung, Younju; Kang, Hyunju; Moon, Jeong; Jang, Hyowon; Park, Sohyun; Kwon, Hyung Jun; Lee, In Chul; Kim, Sunjoo; Yong, Dongeun; Yoon, Sun Woo; Park, Sung Gyu; Guk, Kyeonghye; Lim, Eun Kyung; Park, Hyun Gyu; Choo, Jaebum; Jung, Juyeon; Kang, Taejoon.

In: Biosensors and Bioelectronics, Vol. 202, 114008, 15.04.2022.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

AU - Cha, Hyunjung

AU - Kim, Hyeran

AU - Joung, Younju

AU - Kang, Hyunju

AU - Moon, Jeong

AU - Jang, Hyowon

AU - Park, Sohyun

AU - Kwon, Hyung Jun

AU - Lee, In Chul

AU - Kim, Sunjoo

AU - Yong, Dongeun

AU - Yoon, Sun Woo

AU - Park, Sung Gyu

AU - Guk, Kyeonghye

AU - Lim, Eun Kyung

AU - Park, Hyun Gyu

AU - Choo, Jaebum

AU - Jung, Juyeon

AU - Kang, Taejoon

N1 - Funding Information: This work was supported by the National R&D Programs through the National Research Foundation (NRF) of Korea funded by the Ministry of Science and ICT (MSIT) of Korea [ NRF-2021M3E5E3080379 , NRF-2021M3E5E3080382 , NRF-2021M3H4A1A02051048 , NRF-2018M3A9E2022821 , NRF-2019R1A2C3004375 , NRF-2020R1A5A1018052 , NRF-2020R1A2C1010453 , and NRF-2021M3E5E3080844 ]; the Global Frontier Program through the Center for BioNano Health Guard funded by the MSIT of Korea [ H-GUARD_2013M3A6B2078950 and H-GUARD_2014M3A6B2060507 ]; the National Research Council of Science & Technology (NST) grant by the MSIT of Korea [ CRC21021-100 ]; the Technology Development Program for Biological Hazards Management in Indoor Air through the Korea Environment Industry & Technology Institute (KEITI) funded by the Ministry of Environment (ME) of Korea [ 2021003370003 ]; the Industrial Technology Alchemist Program funded by Ministry of Trade, Industry, and Energy ( MOTIE ) of Korea [ 20012435 ]; the Nanomedical Devices Development Program of the National Nano Fab Center [ CSM2105M101 ]; and the KRIBB Research Initiative Program [ 1711134081 ]. The funding source played no role in study design; in the collection, analysis, and interpretation of data; in the writing of the report; or the decision to submit the article for publication. Publisher Copyright: © 2022 The Authors

PY - 2022/4/15

Y1 - 2022/4/15

N2 - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed for the detection of SARS-CoV-2, advanced sensing methods are required for the diagnosis, screening, and surveillance of coronavirus disease 2019 (COVID-19). Here, we report a surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2. The selected antibody pair against the SARS-CoV-2 antigen, along with the magnetic beads, facilitates the accurate direct detection of the virus. The hollow Au NPs exhibit strong, reproducible SERS signals, allowing sensitive quantitative detection of SARS-CoV-2. This assay had detection limits of 2.56 fg/mL for the SARS-CoV-2 antigen and 3.4 plaque-forming units/mL for the SARS-CoV-2 lysates. Furthermore, it facilitated the identification of SARS-CoV-2 in human nasopharyngeal aspirates and diagnosis of COVID-19 within 30 min using a portable Raman device. Thus, this assay can be potentially used for the diagnosis and prevention of COVID-19.

AB - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed for the detection of SARS-CoV-2, advanced sensing methods are required for the diagnosis, screening, and surveillance of coronavirus disease 2019 (COVID-19). Here, we report a surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2. The selected antibody pair against the SARS-CoV-2 antigen, along with the magnetic beads, facilitates the accurate direct detection of the virus. The hollow Au NPs exhibit strong, reproducible SERS signals, allowing sensitive quantitative detection of SARS-CoV-2. This assay had detection limits of 2.56 fg/mL for the SARS-CoV-2 antigen and 3.4 plaque-forming units/mL for the SARS-CoV-2 lysates. Furthermore, it facilitated the identification of SARS-CoV-2 in human nasopharyngeal aspirates and diagnosis of COVID-19 within 30 min using a portable Raman device. Thus, this assay can be potentially used for the diagnosis and prevention of COVID-19.

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JO - Biosensors and Bioelectronics

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Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

Abstract

Bibliographical note

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UN SDGs

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