To date, the application of RNA therapeutics to hematologic malignancies has been challenging owing to the resistance of blood cancer cells against conventional transfection methods. Herein, triple-targeting moiety-functionalized polymeric small interfering RNA (siRNA) nanoparticles were systematically developed for efficient targeted delivery of RNA therapeutics to hematologic cancer cells. Polymeric siRNAs were synthesized using rolling circle transcription and were surface-functionalized with three types of targeting moieties─a natural ligand and two additional combinations of cell-specific antibodies─for tunable targetability. As a proof of concept, the optimization of the hyaluronic acid/antibody conjugation ratio was performed for selective intracellular delivery to various non-Hodgkin's lymphoma (NHL) cell lines (Daudi, Raji, Ramos, and Toledo cells) via receptor-mediated endocytosis. The engineered nanoparticles showed almost 10-fold enhanced NHL-specific intracellular delivery and induced significant in vitro anticancer effects. This multitargeted nanoparticle platform may effectively support the intracellular delivery of polymeric siRNA sequences, and thus promote therapeutic effects in hematopoietic malignancies.
|Number of pages||9|
|Publication status||Published - 2022 Jun 13|
Bibliographical noteFunding Information:
This work was supported by the Basic Science Research Program (no. 2018R1D1A1A02085552, no. 2020M3F7A1094089, no. 2021R1A6A3A13044758, and no. 2021R1I1A1A01060428) through the National Research Foundation of Korea (NRF) funded by the Korean Government and in part by the Brain Korea 21 (BK21) FOUR program. K.Y. was supported by the NRF-2017-Global Ph.D. Fellowship program.
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All Science Journal Classification (ASJC) codes
- Polymers and Plastics
- Materials Chemistry