Surface localization, regulation, and biologic properties of the 96-kDa alcohol/ aldehyde dehydrogenase (EhADH2) of pathogenic Entamoeba histolytica

Becky M. Flores, Samuel L. Stanley, Taisoon Yong, Manzoor Ali, Wengang Yang, Dana L. Diedrich, Bruce E. Torian

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The 96-kDa surface antigen of Entamoeba histolytica was demonstrated through extensive immunologic evaluation with monoclonal and monospecific antibodies to be identical to or an isoform of the amebic alcohol/aldehyde dehydrogenase (EhADH2). EhADH2 was secreted, excreted, or shed into the culture medium in quantities commensurate with amebic growth when studied in a novel culture system. Of importance, using RNase protection assays, specific mRNA coding for the EhADH2 gene product(s) was up-regulated by treatment of viable trophozoites with the enzyme substrate ethanol. These data provide insight into the biology of this enzyme and its regulation by appropriate stressors.

Original languageEnglish
Pages (from-to)226-231
Number of pages6
JournalJournal of Infectious Diseases
Volume173
Issue number1
DOIs
Publication statusPublished - 1996 Jan 1

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Aldehyde Dehydrogenase
Entamoeba histolytica
Alcohol Dehydrogenase
Trophozoites
Enzymes
Surface Antigens
Ribonucleases
Culture Media
Protein Isoforms
Ethanol
Monoclonal Antibodies
Messenger RNA
Growth
Genes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Flores, Becky M. ; Stanley, Samuel L. ; Yong, Taisoon ; Ali, Manzoor ; Yang, Wengang ; Diedrich, Dana L. ; Torian, Bruce E. / Surface localization, regulation, and biologic properties of the 96-kDa alcohol/ aldehyde dehydrogenase (EhADH2) of pathogenic Entamoeba histolytica. In: Journal of Infectious Diseases. 1996 ; Vol. 173, No. 1. pp. 226-231.
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Surface localization, regulation, and biologic properties of the 96-kDa alcohol/ aldehyde dehydrogenase (EhADH2) of pathogenic Entamoeba histolytica. / Flores, Becky M.; Stanley, Samuel L.; Yong, Taisoon; Ali, Manzoor; Yang, Wengang; Diedrich, Dana L.; Torian, Bruce E.

In: Journal of Infectious Diseases, Vol. 173, No. 1, 01.01.1996, p. 226-231.

Research output: Contribution to journalArticle

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