In this work, we demonstrate that parylene-N can improve the immobilization efficiency of proteins through a UV-treatment. The UV-treatment can be carried out under atmospheric conditions at room temperature without the need for the gas control and vacuum conditions that are required for plasma-treatment. Additionally, the UV-treatment does not require the collision of high-energy gas molecules; the surface roughness of parylene-N is maintained throughout the modification process. To characterize the physical and chemical properties of the UV-treated parylene-N, contact angle (advancing and receding), grazing angle FT-IR spectroscopy (for thin films), and X-ray photoelectron spectroscopy (for depth profiling) measurements were carried out. The protein immobilization efficiency of the UV-treated parylene-N was estimated with SPR biosensors using proteins with different surface charges, and the long-term stability of the protein immobilization capabilities were also estimated as compared with plasma-treated parylene-N.
Bibliographical noteFunding Information:
This research was supported by the National Research Foundation of Korea ( 2012R1A2A2A03047461 , 2011-0020285 , 2014M3A9E5073818 and 2013R1A1A2065723 ). This work was also supported by the Creative Fusion Research Program through the Creative Allied Project funded by the Korea Research Council of Fundamental Science and Technology ( CAP-12-1 ).
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All Science Journal Classification (ASJC) codes
- Physics and Astronomy(all)
- Organic Chemistry
- Polymers and Plastics
- Materials Chemistry