Surface modification of parylene-N with UV-treatment to enhance the protein immobilization

Hyuk Ko, Yong Hwan Choi, Seo Yoon Chang, Ga Yeon Lee, Hyun Woo Song, Young Wook Chang, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In this work, we demonstrate that parylene-N can improve the immobilization efficiency of proteins through a UV-treatment. The UV-treatment can be carried out under atmospheric conditions at room temperature without the need for the gas control and vacuum conditions that are required for plasma-treatment. Additionally, the UV-treatment does not require the collision of high-energy gas molecules; the surface roughness of parylene-N is maintained throughout the modification process. To characterize the physical and chemical properties of the UV-treated parylene-N, contact angle (advancing and receding), grazing angle FT-IR spectroscopy (for thin films), and X-ray photoelectron spectroscopy (for depth profiling) measurements were carried out. The protein immobilization efficiency of the UV-treated parylene-N was estimated with SPR biosensors using proteins with different surface charges, and the long-term stability of the protein immobilization capabilities were also estimated as compared with plasma-treated parylene-N.

Original languageEnglish
Pages (from-to)36-46
Number of pages11
JournalEuropean Polymer Journal
Volume68
DOIs
Publication statusPublished - 2015 Jul 1

Fingerprint

immobilization
Surface treatment
proteins
Proteins
Plasmas
Gases
Depth profiling
grazing
meteorology
Surface charge
bioinstrumentation
gases
Biosensors
chemical properties
Chemical properties
Contact angle
Infrared spectroscopy
surface roughness
X ray photoelectron spectroscopy
Physical properties

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Ko, Hyuk ; Choi, Yong Hwan ; Chang, Seo Yoon ; Lee, Ga Yeon ; Song, Hyun Woo ; Chang, Young Wook ; Kang, Min Jung ; Pyun, Jae Chul. / Surface modification of parylene-N with UV-treatment to enhance the protein immobilization. In: European Polymer Journal. 2015 ; Vol. 68. pp. 36-46.
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Surface modification of parylene-N with UV-treatment to enhance the protein immobilization. / Ko, Hyuk; Choi, Yong Hwan; Chang, Seo Yoon; Lee, Ga Yeon; Song, Hyun Woo; Chang, Young Wook; Kang, Min Jung; Pyun, Jae Chul.

In: European Polymer Journal, Vol. 68, 01.07.2015, p. 36-46.

Research output: Contribution to journalArticle

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