Surface plasmon enhanced cell microscopy with blocked random spatial activation

Taehwang Son; Youngjin Oh; Wonju Lee; Heejin Yang; Donghyun Kim

doi:10.1117/12.2210753

Surface plasmon enhanced cell microscopy with blocked random spatial activation

Taehwang Son, Youngjin Oh, Wonju Lee, Heejin Yang, Donghyun Kim

Department of Electrical and Electronic Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We present surface plasmon enhanced fluorescence microscopy with random spatial sampling using patterned block of silver nanoislands. Rigorous coupled wave analysis was performed to confirm near-field localization on nanoislands. Random nanoislands were fabricated in silver by temperature annealing. By analyzing random near-field distribution, average size of localized fields was found to be on the order of 135 nm. Randomly localized near-fields were used to spatially sample F-actin of J774 cells (mouse macrophage cell-line). Image deconvolution algorithm based on linear imaging theory was established for stochastic estimation of fluorescent molecular distribution. The alignment between near-field distribution and raw image was performed by the patterned block. The achieved resolution is dependent upon factors including the size of localized fields and estimated to be 100-150 nm.

Original language	English
Title of host publication	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
Editors	Alexander N. Cartwright, Dan V. Nicolau
Publisher	SPIE
ISBN (Electronic)	9781628419559
DOIs	https://doi.org/10.1117/12.2210753
Publication status	Published - 2016
Event	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII - San Francisco, United States Duration: 2016 Feb 15 → 2016 Feb 17

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9721
ISSN (Print)	1605-7422

Other

Other	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
Country/Territory	United States
City	San Francisco
Period	16/2/15 → 16/2/17

Bibliographical note

Publisher Copyright:
© 2016 SPIE.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

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10.1117/12.2210753

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Son, T., Oh, Y., Lee, W., Yang, H., & Kim, D. (2016). Surface plasmon enhanced cell microscopy with blocked random spatial activation. In A. N. Cartwright, & D. V. Nicolau (Eds.), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII [97210A] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9721). SPIE. https://doi.org/10.1117/12.2210753

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abstract = "We present surface plasmon enhanced fluorescence microscopy with random spatial sampling using patterned block of silver nanoislands. Rigorous coupled wave analysis was performed to confirm near-field localization on nanoislands. Random nanoislands were fabricated in silver by temperature annealing. By analyzing random near-field distribution, average size of localized fields was found to be on the order of 135 nm. Randomly localized near-fields were used to spatially sample F-actin of J774 cells (mouse macrophage cell-line). Image deconvolution algorithm based on linear imaging theory was established for stochastic estimation of fluorescent molecular distribution. The alignment between near-field distribution and raw image was performed by the patterned block. The achieved resolution is dependent upon factors including the size of localized fields and estimated to be 100-150 nm.",

author = "Taehwang Son and Youngjin Oh and Wonju Lee and Heejin Yang and Donghyun Kim",

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Son, T, Oh, Y, Lee, W, Yang, H & Kim, D 2016, Surface plasmon enhanced cell microscopy with blocked random spatial activation. in AN Cartwright & DV Nicolau (eds), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII., 97210A, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9721, SPIE, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, San Francisco, United States, 16/2/15. https://doi.org/10.1117/12.2210753

Surface plasmon enhanced cell microscopy with blocked random spatial activation. / Son, Taehwang; Oh, Youngjin; Lee, Wonju; Yang, Heejin; Kim, Donghyun.

Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. ed. / Alexander N. Cartwright; Dan V. Nicolau. SPIE, 2016. 97210A (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9721).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Yang, Heejin

AU - Kim, Donghyun

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N2 - We present surface plasmon enhanced fluorescence microscopy with random spatial sampling using patterned block of silver nanoislands. Rigorous coupled wave analysis was performed to confirm near-field localization on nanoislands. Random nanoislands were fabricated in silver by temperature annealing. By analyzing random near-field distribution, average size of localized fields was found to be on the order of 135 nm. Randomly localized near-fields were used to spatially sample F-actin of J774 cells (mouse macrophage cell-line). Image deconvolution algorithm based on linear imaging theory was established for stochastic estimation of fluorescent molecular distribution. The alignment between near-field distribution and raw image was performed by the patterned block. The achieved resolution is dependent upon factors including the size of localized fields and estimated to be 100-150 nm.

AB - We present surface plasmon enhanced fluorescence microscopy with random spatial sampling using patterned block of silver nanoislands. Rigorous coupled wave analysis was performed to confirm near-field localization on nanoislands. Random nanoislands were fabricated in silver by temperature annealing. By analyzing random near-field distribution, average size of localized fields was found to be on the order of 135 nm. Randomly localized near-fields were used to spatially sample F-actin of J774 cells (mouse macrophage cell-line). Image deconvolution algorithm based on linear imaging theory was established for stochastic estimation of fluorescent molecular distribution. The alignment between near-field distribution and raw image was performed by the patterned block. The achieved resolution is dependent upon factors including the size of localized fields and estimated to be 100-150 nm.

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BT - Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII

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Surface plasmon enhanced cell microscopy with blocked random spatial activation

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