Abstract
We present surface plasmon enhanced fluorescence microscopy with random spatial sampling using patterned block of silver nanoislands. Rigorous coupled wave analysis was performed to confirm near-field localization on nanoislands. Random nanoislands were fabricated in silver by temperature annealing. By analyzing random near-field distribution, average size of localized fields was found to be on the order of 135 nm. Randomly localized near-fields were used to spatially sample F-actin of J774 cells (mouse macrophage cell-line). Image deconvolution algorithm based on linear imaging theory was established for stochastic estimation of fluorescent molecular distribution. The alignment between near-field distribution and raw image was performed by the patterned block. The achieved resolution is dependent upon factors including the size of localized fields and estimated to be 100-150 nm.
| Original language | English |
|---|---|
| Title of host publication | Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII |
| Publisher | SPIE |
| Volume | 9721 |
| ISBN (Electronic) | 9781628419559 |
| DOIs | |
| Publication status | Published - 2016 Jan 1 |
| Event | Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII - San Francisco, United States Duration: 2016 Feb 15 → 2016 Feb 17 |
Other
| Other | Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII |
|---|---|
| Country | United States |
| City | San Francisco |
| Period | 16/2/15 → 16/2/17 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging
Cite this
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Surface plasmon enhanced cell microscopy with blocked random spatial activation. / Son, Taehwang; Oh, Youngjin; Lee, Wonju; Yang, Heejin; Kim, Donghyun.
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. Vol. 9721 SPIE, 2016. 97210A.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Surface plasmon enhanced cell microscopy with blocked random spatial activation
AU - Son, Taehwang
AU - Oh, Youngjin
AU - Lee, Wonju
AU - Yang, Heejin
AU - Kim, Donghyun
PY - 2016/1/1
Y1 - 2016/1/1
N2 - We present surface plasmon enhanced fluorescence microscopy with random spatial sampling using patterned block of silver nanoislands. Rigorous coupled wave analysis was performed to confirm near-field localization on nanoislands. Random nanoislands were fabricated in silver by temperature annealing. By analyzing random near-field distribution, average size of localized fields was found to be on the order of 135 nm. Randomly localized near-fields were used to spatially sample F-actin of J774 cells (mouse macrophage cell-line). Image deconvolution algorithm based on linear imaging theory was established for stochastic estimation of fluorescent molecular distribution. The alignment between near-field distribution and raw image was performed by the patterned block. The achieved resolution is dependent upon factors including the size of localized fields and estimated to be 100-150 nm.
AB - We present surface plasmon enhanced fluorescence microscopy with random spatial sampling using patterned block of silver nanoislands. Rigorous coupled wave analysis was performed to confirm near-field localization on nanoislands. Random nanoislands were fabricated in silver by temperature annealing. By analyzing random near-field distribution, average size of localized fields was found to be on the order of 135 nm. Randomly localized near-fields were used to spatially sample F-actin of J774 cells (mouse macrophage cell-line). Image deconvolution algorithm based on linear imaging theory was established for stochastic estimation of fluorescent molecular distribution. The alignment between near-field distribution and raw image was performed by the patterned block. The achieved resolution is dependent upon factors including the size of localized fields and estimated to be 100-150 nm.
UR - http://www.scopus.com/inward/record.url?scp=84982167189&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84982167189&partnerID=8YFLogxK
U2 - 10.1117/12.2210753
DO - 10.1117/12.2210753
M3 - Conference contribution
AN - SCOPUS:84982167189
VL - 9721
BT - Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
PB - SPIE
ER -