Surface plasmon enhanced cell microscopy with blocked random spatial activation

Taehwang Son, Youngjin Oh, Wonju Lee, Heejin Yang, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present surface plasmon enhanced fluorescence microscopy with random spatial sampling using patterned block of silver nanoislands. Rigorous coupled wave analysis was performed to confirm near-field localization on nanoislands. Random nanoislands were fabricated in silver by temperature annealing. By analyzing random near-field distribution, average size of localized fields was found to be on the order of 135 nm. Randomly localized near-fields were used to spatially sample F-actin of J774 cells (mouse macrophage cell-line). Image deconvolution algorithm based on linear imaging theory was established for stochastic estimation of fluorescent molecular distribution. The alignment between near-field distribution and raw image was performed by the patterned block. The achieved resolution is dependent upon factors including the size of localized fields and estimated to be 100-150 nm.

Original languageEnglish
Title of host publicationNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
PublisherSPIE
Volume9721
ISBN (Electronic)9781628419559
DOIs
Publication statusPublished - 2016 Jan 1
EventNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII - San Francisco, United States
Duration: 2016 Feb 152016 Feb 17

Other

OtherNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
CountryUnited States
CitySan Francisco
Period16/2/1516/2/17

Fingerprint

Silver
Microscopy
near fields
Microscopic examination
Chemical activation
activation
microscopy
Macrophages
Fluorescence microscopy
Deconvolution
cells
Fluorescence Microscopy
Actins
silver
Annealing
Sampling
Imaging techniques
Cell Line
Temperature
macrophages

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Son, T., Oh, Y., Lee, W., Yang, H., & Kim, D. (2016). Surface plasmon enhanced cell microscopy with blocked random spatial activation. In Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII (Vol. 9721). [97210A] SPIE. https://doi.org/10.1117/12.2210753
Son, Taehwang ; Oh, Youngjin ; Lee, Wonju ; Yang, Heejin ; Kim, Donghyun. / Surface plasmon enhanced cell microscopy with blocked random spatial activation. Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. Vol. 9721 SPIE, 2016.
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Son, T, Oh, Y, Lee, W, Yang, H & Kim, D 2016, Surface plasmon enhanced cell microscopy with blocked random spatial activation. in Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. vol. 9721, 97210A, SPIE, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, San Francisco, United States, 16/2/15. https://doi.org/10.1117/12.2210753

Surface plasmon enhanced cell microscopy with blocked random spatial activation. / Son, Taehwang; Oh, Youngjin; Lee, Wonju; Yang, Heejin; Kim, Donghyun.

Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. Vol. 9721 SPIE, 2016. 97210A.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - We present surface plasmon enhanced fluorescence microscopy with random spatial sampling using patterned block of silver nanoislands. Rigorous coupled wave analysis was performed to confirm near-field localization on nanoislands. Random nanoislands were fabricated in silver by temperature annealing. By analyzing random near-field distribution, average size of localized fields was found to be on the order of 135 nm. Randomly localized near-fields were used to spatially sample F-actin of J774 cells (mouse macrophage cell-line). Image deconvolution algorithm based on linear imaging theory was established for stochastic estimation of fluorescent molecular distribution. The alignment between near-field distribution and raw image was performed by the patterned block. The achieved resolution is dependent upon factors including the size of localized fields and estimated to be 100-150 nm.

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Son T, Oh Y, Lee W, Yang H, Kim D. Surface plasmon enhanced cell microscopy with blocked random spatial activation. In Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. Vol. 9721. SPIE. 2016. 97210A https://doi.org/10.1117/12.2210753