Surface plasmon enhanced cell microscopy with blocked random spatial activation

Taehwang Son, Youngjin Oh, Wonju Lee, Heejin Yang, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present surface plasmon enhanced fluorescence microscopy with random spatial sampling using patterned block of silver nanoislands. Rigorous coupled wave analysis was performed to confirm near-field localization on nanoislands. Random nanoislands were fabricated in silver by temperature annealing. By analyzing random near-field distribution, average size of localized fields was found to be on the order of 135 nm. Randomly localized near-fields were used to spatially sample F-actin of J774 cells (mouse macrophage cell-line). Image deconvolution algorithm based on linear imaging theory was established for stochastic estimation of fluorescent molecular distribution. The alignment between near-field distribution and raw image was performed by the patterned block. The achieved resolution is dependent upon factors including the size of localized fields and estimated to be 100-150 nm.

Original languageEnglish
Title of host publicationNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
PublisherSPIE
Volume9721
ISBN (Electronic)9781628419559
DOIs
Publication statusPublished - 2016 Jan 1
EventNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII - San Francisco, United States
Duration: 2016 Feb 152016 Feb 17

Other

OtherNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
CountryUnited States
CitySan Francisco
Period16/2/1516/2/17

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Son, T., Oh, Y., Lee, W., Yang, H., & Kim, D. (2016). Surface plasmon enhanced cell microscopy with blocked random spatial activation. In Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII (Vol. 9721). [97210A] SPIE. https://doi.org/10.1117/12.2210753