We present surface plasmon enhanced fluorescence microscopy with random spatial sampling using patterned block of silver nanoislands. Rigorous coupled wave analysis was performed to confirm near-field localization on nanoislands. Random nanoislands were fabricated in silver by temperature annealing. By analyzing random near-field distribution, average size of localized fields was found to be on the order of 135 nm. Randomly localized near-fields were used to spatially sample F-actin of J774 cells (mouse macrophage cell-line). Image deconvolution algorithm based on linear imaging theory was established for stochastic estimation of fluorescent molecular distribution. The alignment between near-field distribution and raw image was performed by the patterned block. The achieved resolution is dependent upon factors including the size of localized fields and estimated to be 100-150 nm.
|Title of host publication||Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII|
|Editors||Alexander N. Cartwright, Dan V. Nicolau|
|Publication status||Published - 2016|
|Event||Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII - San Francisco, United States|
Duration: 2016 Feb 15 → 2016 Feb 17
|Name||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|Other||Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII|
|Period||16/2/15 → 16/2/17|
Bibliographical notePublisher Copyright:
© 2016 SPIE.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging