Surface plasmon-enhanced nanoscopy of intracellular cytoskeletal actin filaments using random nanodot arrays

Youngjin Oh; Taehwang Son; Sook Young Kim; Wonju Lee; Heejin Yang; Jong Ryul Choi; Jeon Soo Shin; Donghyun Kim

doi:10.1364/OE.22.027695

Surface plasmon-enhanced nanoscopy of intracellular cytoskeletal actin filaments using random nanodot arrays

Youngjin Oh, Taehwang Son, Sook Young Kim, Wonju Lee, Heejin Yang, Jong Ryul Choi, Jeon Soo Shin, Donghyun Kim

Department of Electrical and Electronic Engineering

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

The feasibility of super-resolution microscopy has been investigated based on random localization of surface plasmon using blocked random nanodot arrays. The resolution is mainly determined by the size of localized fields in the range of 100-150 nm. The concept was validated by imaging FITC-conjugated phalloidin that binds to cellular actin filaments. The experimental results confirm improved resolution in reconstructed images. Effect of far-field registration on image reconstruction was also analyzed. Correlation between reconstructed images was maintained to be above 81% after registration. Nanodot arrays are synthesized by temperature-annealing without sophisticated lithography and thus can be mass-produced in an extremely large substrate. The results suggest a superresolution imaging technique that can be accessible and available in large amounts.

Original language	English
Pages (from-to)	27695-27706
Number of pages	12
Journal	Optics Express
Volume	22
Issue number	22
DOIs	https://doi.org/10.1364/OE.22.027695
Publication status	Published - 2014

Bibliographical note

Publisher Copyright:
©2014 Optical Society of America

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

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10.1364/OE.22.027695

Cite this

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title = "Surface plasmon-enhanced nanoscopy of intracellular cytoskeletal actin filaments using random nanodot arrays",

abstract = "The feasibility of super-resolution microscopy has been investigated based on random localization of surface plasmon using blocked random nanodot arrays. The resolution is mainly determined by the size of localized fields in the range of 100-150 nm. The concept was validated by imaging FITC-conjugated phalloidin that binds to cellular actin filaments. The experimental results confirm improved resolution in reconstructed images. Effect of far-field registration on image reconstruction was also analyzed. Correlation between reconstructed images was maintained to be above 81% after registration. Nanodot arrays are synthesized by temperature-annealing without sophisticated lithography and thus can be mass-produced in an extremely large substrate. The results suggest a superresolution imaging technique that can be accessible and available in large amounts.",

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AU - Oh, Youngjin

AU - Son, Taehwang

AU - Kim, Sook Young

AU - Lee, Wonju

AU - Yang, Heejin

AU - Choi, Jong Ryul

AU - Shin, Jeon Soo

AU - Kim, Donghyun

PY - 2014

Y1 - 2014

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Surface plasmon-enhanced nanoscopy of intracellular cytoskeletal actin filaments using random nanodot arrays

Abstract

Bibliographical note

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