Surrogate reporters for enrichment of cells with nuclease-induced mutations

Hyojin Kim, Eunji Um, Sung Rae Cho, Chorong Jung, Hyongbum Kim, Jin Soo Kim

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Zinc-finger nucleases (ZFNs) and TAL-effector nucleases (TALENs) are powerful tools for creating genetic modifications in eukaryotic cells and organisms. But wild-type and mutant cells that contain genetic modifications induced by these programmable nucleases are often phenotypically indistinguishable, hampering isolation of mutant cells. Here we show that transiently transfected episomal reporters encoding fluorescent proteins can be used as surrogate genes for the efficient enrichment of endogenous gene-modified cells by flow cytometry.

Original languageEnglish
Pages (from-to)941-943
Number of pages3
JournalNature Methods
Volume8
Issue number11
DOIs
Publication statusPublished - 2011 Nov

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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