Suspension arrays of hydrogel microparticles prepared by photopatterning for multiplexed protein-based bioassays

Woojin Lee, Dongkil Choi, Jung Hyun Kim, Won Gun Koh

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Suspension arrays for protein-based assays have been developed using shape-coded poly(ethylene glycol) (PEG) hydrogel microparticles to overcome the problems with current systems which use color-coded rigid microparticles as protein supports. Various shapes of hydrogel microparticles were fabricated by a two-step process consisting of photopatterning and flushing using a poly(dimethylsiloxane) (PDMS) channel as a molding insert. Hydrogel microparticles with lateral dimensions ranging from 50 to 300 μm were fabricated using different molecular weights of PEG (700, 3,400, and 8,000 Da), by which the water content and swelling behavior of the hydrogel microparticles could be controlled. Protein-entrapped hydrogel microparticles were prepared in a suspension array format, and PEG hydrogel could encapsulate proteins without deactivation for a week due to its high water content and soft nature. The sequential bienzymatic reaction of hydrogel-entrapped glucose oxidase (GOX) and peroxidase (POD) was successfully investigated using fluorescence detection, demonstrating one possible application of suspension arrays. Furthermore, a mixture of two different shapes of hydrogel microparticles containing GOX/POD and alkaline phosphatase (AP), respectively, was prepared and the shape-coded suspension array was used for simultaneous characterization of two different enzyme-catalyzed reactions.

Original languageEnglish
Pages (from-to)813-822
Number of pages10
JournalBiomedical Microdevices
Volume10
Issue number6
DOIs
Publication statusPublished - 2008 Jun 18

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Bioassay
Hydrogel
Hydrogels
Biological Assay
Suspensions
Proteins
Polyethylene glycols
Glucose Oxidase
Glucose oxidase
Water content
Protein Array Analysis
Die casting inserts
Ethylene Glycol
Water
Phosphatases
Polydimethylsiloxane
Molding
Alkaline Phosphatase
Swelling
Assays

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Molecular Biology

Cite this

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abstract = "Suspension arrays for protein-based assays have been developed using shape-coded poly(ethylene glycol) (PEG) hydrogel microparticles to overcome the problems with current systems which use color-coded rigid microparticles as protein supports. Various shapes of hydrogel microparticles were fabricated by a two-step process consisting of photopatterning and flushing using a poly(dimethylsiloxane) (PDMS) channel as a molding insert. Hydrogel microparticles with lateral dimensions ranging from 50 to 300 μm were fabricated using different molecular weights of PEG (700, 3,400, and 8,000 Da), by which the water content and swelling behavior of the hydrogel microparticles could be controlled. Protein-entrapped hydrogel microparticles were prepared in a suspension array format, and PEG hydrogel could encapsulate proteins without deactivation for a week due to its high water content and soft nature. The sequential bienzymatic reaction of hydrogel-entrapped glucose oxidase (GOX) and peroxidase (POD) was successfully investigated using fluorescence detection, demonstrating one possible application of suspension arrays. Furthermore, a mixture of two different shapes of hydrogel microparticles containing GOX/POD and alkaline phosphatase (AP), respectively, was prepared and the shape-coded suspension array was used for simultaneous characterization of two different enzyme-catalyzed reactions.",
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Suspension arrays of hydrogel microparticles prepared by photopatterning for multiplexed protein-based bioassays. / Lee, Woojin; Choi, Dongkil; Kim, Jung Hyun; Koh, Won Gun.

In: Biomedical Microdevices, Vol. 10, No. 6, 18.06.2008, p. 813-822.

Research output: Contribution to journalArticle

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