Syndecan-2 functions as a docking receptor for pro-matrix metalloproteinase-7 in human colon cancer cells

Heui Young Ryu, Jiseon Lee, Sanghwa Yang, Haein Park, Sojoong Choi, Kyeong Cheon Jung, Seung Taek Lee, Je Kyung Seong, Inn Oc Han, Eok Soo Oh

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Abstract

Although elevated syndecan-2 expression is known to be crucial for the tumorigenic activity in colon carcinoma cells, how syndecan-2 regulates colon cancer is unclear. In human colon adenocarcinoma tissue samples, we found that both mRNA and protein expression of syndecan-2 were increased, compared with the neighboring normal epithelium, suggesting that syndecan-2 plays functional roles in human colon cancer cells. Consistent with this notion, syndecan-2-overexpressing HT-29 colon adenocarcinoma cells showed enhanced migration/invasion, anchorage-independent growth, and primary tumor formation in nude mice, paralleling their morphological changes into highly tumorigenic cells. In addition, our experiments revealed that syndecan-2 enhanced both expression and secretion of matrix metalloproteinase-7 (MMP-7), directly interacted with pro-MMP-7, and potentiated the enzymatic activity of pro-MMP-7 by activating its processing into the active MMP-7. Collectively, these data strongly suggest that syndecan-2 functions as a docking receptor for pro-MMP-7 in colon cancer cells.

Original languageEnglish
Pages (from-to)35692-35701
Number of pages10
JournalJournal of Biological Chemistry
Volume284
Issue number51
DOIs
Publication statusPublished - 2009 Dec 18

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ryu, H. Y., Lee, J., Yang, S., Park, H., Choi, S., Jung, K. C., Lee, S. T., Seong, J. K., Han, I. O., & Oh, E. S. (2009). Syndecan-2 functions as a docking receptor for pro-matrix metalloproteinase-7 in human colon cancer cells. Journal of Biological Chemistry, 284(51), 35692-35701. https://doi.org/10.1074/jbc.M109.054254