Synergistic Action of IL-8 and Bone Marrow Concentrate on Cartilage Regeneration Through Upregulation of Chondrogenic Transcription Factors

Dong Suk Yoon, Kyoung Mi Lee, Sung Hwan Kim, Su Hee Kim, Youngmee Jung, Soo Hyun Kim, Kwang Hwan Park, Yoorim Choi, Hyun Aae Ryu, Woo Jin Choi, jinwoo lee

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The objective of this study was to determine whether a biphasic scaffold loaded with a combination of a chemokine and bone marrow concentrate (BMC) could improve tissue regeneration in knee articular cartilage of beagles with cylindrical osteochondral defects. For this investigation, an osteochondral defect (6 mm in diameter and 8 mm deep) was created in the weight-bearing articular surface of the femoral medial condyle in beagles. Bone marrow was aspirated from the posterior iliac crests of beagles to obtain mesenchymal stem cells (MSCs) for in vitro assay. Hematoxylin and eosin (HE), Masson's trichrome (MT), safranin O/fast green staining, and immunohistochemistry were performed for histological analysis. Quantitative real-time polymerase chain reaction was performed to understand the roles of BMC in chondrogenic differentiation of MSCs. At 12 weeks after transplantation of biphasic scaffolds, we observed that interleukin-8 (IL-8) or the combination of IL-8 and BMC induced massive bone regeneration compared to saline, BMC only, and MSCs. In gross appearance, the osteochondral defect site was nearly completely filled with repair tissue in the group that received the combination of IL-8 and BMC but not in the other groups. Moreover, histological analysis showed obvious differences in cartilage regeneration among groups. HE and MT staining showed that the cartilage defect sites of the group receiving the combination of IL-8 and BMC were regenerated with cartilage-like tissues showing chondrocyte morphology. Safranin O staining showed hyaline cartilage regeneration in the group receiving IL-8 and BMC, whereas fibrous-like tissues were formed in the other groups. Furthermore, immunostaining revealed the presence of type II collagen and aggrecan in regenerated cartilage tissue of the group receiving IL-8 and BMC, whereas regenerated cartilage tissues of the other groups weakly expressed type II collagen and aggrecan. These results indicate that the combination of a chemokine IL-8 and BMC has significant positive effects on osteochondral regeneration in a beagle model through enhancing expression of the chondrogenic transcription factors and markers such as Sox9 and type II collagen.

Original languageEnglish
Pages (from-to)363-374
Number of pages12
JournalTissue Engineering - Part A
Volume22
Issue number3-4
DOIs
Publication statusPublished - 2016 Feb 1

Fingerprint

Transcription factors
Cartilage
Interleukin-8
Regeneration
Bone
Transcription Factors
Up-Regulation
Bone Marrow
Collagen Type II
Tissue
Mesenchymal Stromal Cells
Stem cells
Collagen
Aggrecans
Defects
Hematoxylin
Eosine Yellowish-(YS)
Staining and Labeling
Chemokines
Scaffolds

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Yoon, Dong Suk ; Lee, Kyoung Mi ; Kim, Sung Hwan ; Kim, Su Hee ; Jung, Youngmee ; Kim, Soo Hyun ; Park, Kwang Hwan ; Choi, Yoorim ; Ryu, Hyun Aae ; Choi, Woo Jin ; lee, jinwoo. / Synergistic Action of IL-8 and Bone Marrow Concentrate on Cartilage Regeneration Through Upregulation of Chondrogenic Transcription Factors. In: Tissue Engineering - Part A. 2016 ; Vol. 22, No. 3-4. pp. 363-374.
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abstract = "The objective of this study was to determine whether a biphasic scaffold loaded with a combination of a chemokine and bone marrow concentrate (BMC) could improve tissue regeneration in knee articular cartilage of beagles with cylindrical osteochondral defects. For this investigation, an osteochondral defect (6 mm in diameter and 8 mm deep) was created in the weight-bearing articular surface of the femoral medial condyle in beagles. Bone marrow was aspirated from the posterior iliac crests of beagles to obtain mesenchymal stem cells (MSCs) for in vitro assay. Hematoxylin and eosin (HE), Masson's trichrome (MT), safranin O/fast green staining, and immunohistochemistry were performed for histological analysis. Quantitative real-time polymerase chain reaction was performed to understand the roles of BMC in chondrogenic differentiation of MSCs. At 12 weeks after transplantation of biphasic scaffolds, we observed that interleukin-8 (IL-8) or the combination of IL-8 and BMC induced massive bone regeneration compared to saline, BMC only, and MSCs. In gross appearance, the osteochondral defect site was nearly completely filled with repair tissue in the group that received the combination of IL-8 and BMC but not in the other groups. Moreover, histological analysis showed obvious differences in cartilage regeneration among groups. HE and MT staining showed that the cartilage defect sites of the group receiving the combination of IL-8 and BMC were regenerated with cartilage-like tissues showing chondrocyte morphology. Safranin O staining showed hyaline cartilage regeneration in the group receiving IL-8 and BMC, whereas fibrous-like tissues were formed in the other groups. Furthermore, immunostaining revealed the presence of type II collagen and aggrecan in regenerated cartilage tissue of the group receiving IL-8 and BMC, whereas regenerated cartilage tissues of the other groups weakly expressed type II collagen and aggrecan. These results indicate that the combination of a chemokine IL-8 and BMC has significant positive effects on osteochondral regeneration in a beagle model through enhancing expression of the chondrogenic transcription factors and markers such as Sox9 and type II collagen.",
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Synergistic Action of IL-8 and Bone Marrow Concentrate on Cartilage Regeneration Through Upregulation of Chondrogenic Transcription Factors. / Yoon, Dong Suk; Lee, Kyoung Mi; Kim, Sung Hwan; Kim, Su Hee; Jung, Youngmee; Kim, Soo Hyun; Park, Kwang Hwan; Choi, Yoorim; Ryu, Hyun Aae; Choi, Woo Jin; lee, jinwoo.

In: Tissue Engineering - Part A, Vol. 22, No. 3-4, 01.02.2016, p. 363-374.

Research output: Contribution to journalArticle

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AU - Yoon, Dong Suk

AU - Lee, Kyoung Mi

AU - Kim, Sung Hwan

AU - Kim, Su Hee

AU - Jung, Youngmee

AU - Kim, Soo Hyun

AU - Park, Kwang Hwan

AU - Choi, Yoorim

AU - Ryu, Hyun Aae

AU - Choi, Woo Jin

AU - lee, jinwoo

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