Synergistic effect of bone marrow-derived mesenchymal stem cells and platelet-rich plasma on bone regeneration of calvarial defects in rabbits

Jeong Ho Yun, Jae Heung Yoo, Seongho Choi, Myung Hyun Lee, Sang Jin Lee, Sun U. Song, Nam Sik Oh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Bone tissue regeneration techniques include tissue engineering approaches which employ mesenchymal stem cells as an osteogenic agent for bone repair. Recent studies have demonstrated that tissue engineering scaffolds and growth factors can support cell proliferation, bone formation, and bone tissue repair in lost bone tissue. Furthermore, many studies have suggested that platelet-rich plasma (PRP) can improve bone regeneration due to the numerous growth factors that it contains. This study was performed to investigate the influence of bone marrow-derived mesenchymal stem cells (BMMSCs) and PRP on bone regeneration of calvarial defects in rabbits. Hydroxyapatite (HA) was used as a scaffold for bone regeneration. There were three groups in this experiment: 1) HA loaded with BMMSCs (HS group), 2) HA loaded with PRP (HP group), and 3) HA loaded with BMMSCs and PRP (HSP group). Two circular bony defects (6 mm in diameter) were made in rabbit calvaria using a trephine bur. BMMSCs and PRP with a HA scaffold (diameter 5.5 mm, height 3 mm) were applied to each defect. The animals were sacrificed after 2 weeks, 4 weeks and 8 weeks. The level of their ability of osteogenesis was evaluated through histological and histomorphometric analyses. High-quality bone regeneration was observed in the HSP group. The percentage of new bone area around the scaffolds was higher in the HSP group than it was in the other groups (HS and HP group), especially at 8 weeks (HS, 72.5±15 %; HP, 85.8±14 %; HSP, 98.8±2.5%). In addition, the level of bone maturation was higher in the HSP group than in the other groups. The results of this study show that PRP has a positive effect on bone generation. HA with a combination of BMMSCs and PRP can enhance bone regeneration. In addition, the growth factor capacity of PRP may affect the differentiation of BMMSCs and promote bone formation.

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalTissue Engineering and Regenerative Medicine
Volume9
Issue number1
DOIs
Publication statusPublished - 2012 Feb 1

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Bone Regeneration
Platelet-Rich Plasma
Platelets
Stem cells
Mesenchymal Stromal Cells
Bone
Durapatite
Bone Marrow
Rabbits
Plasmas
Defects
Bone and Bones
Osteogenesis
Intercellular Signaling Peptides and Proteins
Hydroxyapatite
Tissue Engineering
Tissue Scaffolds
Scaffolds
Skull
Tissue engineering

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Yun, Jeong Ho ; Yoo, Jae Heung ; Choi, Seongho ; Lee, Myung Hyun ; Lee, Sang Jin ; Song, Sun U. ; Oh, Nam Sik. / Synergistic effect of bone marrow-derived mesenchymal stem cells and platelet-rich plasma on bone regeneration of calvarial defects in rabbits. In: Tissue Engineering and Regenerative Medicine. 2012 ; Vol. 9, No. 1. pp. 17-23.
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Synergistic effect of bone marrow-derived mesenchymal stem cells and platelet-rich plasma on bone regeneration of calvarial defects in rabbits. / Yun, Jeong Ho; Yoo, Jae Heung; Choi, Seongho; Lee, Myung Hyun; Lee, Sang Jin; Song, Sun U.; Oh, Nam Sik.

In: Tissue Engineering and Regenerative Medicine, Vol. 9, No. 1, 01.02.2012, p. 17-23.

Research output: Contribution to journalArticle

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AU - Yun, Jeong Ho

AU - Yoo, Jae Heung

AU - Choi, Seongho

AU - Lee, Myung Hyun

AU - Lee, Sang Jin

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AU - Oh, Nam Sik

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AB - Bone tissue regeneration techniques include tissue engineering approaches which employ mesenchymal stem cells as an osteogenic agent for bone repair. Recent studies have demonstrated that tissue engineering scaffolds and growth factors can support cell proliferation, bone formation, and bone tissue repair in lost bone tissue. Furthermore, many studies have suggested that platelet-rich plasma (PRP) can improve bone regeneration due to the numerous growth factors that it contains. This study was performed to investigate the influence of bone marrow-derived mesenchymal stem cells (BMMSCs) and PRP on bone regeneration of calvarial defects in rabbits. Hydroxyapatite (HA) was used as a scaffold for bone regeneration. There were three groups in this experiment: 1) HA loaded with BMMSCs (HS group), 2) HA loaded with PRP (HP group), and 3) HA loaded with BMMSCs and PRP (HSP group). Two circular bony defects (6 mm in diameter) were made in rabbit calvaria using a trephine bur. BMMSCs and PRP with a HA scaffold (diameter 5.5 mm, height 3 mm) were applied to each defect. The animals were sacrificed after 2 weeks, 4 weeks and 8 weeks. The level of their ability of osteogenesis was evaluated through histological and histomorphometric analyses. High-quality bone regeneration was observed in the HSP group. The percentage of new bone area around the scaffolds was higher in the HSP group than it was in the other groups (HS and HP group), especially at 8 weeks (HS, 72.5±15 %; HP, 85.8±14 %; HSP, 98.8±2.5%). In addition, the level of bone maturation was higher in the HSP group than in the other groups. The results of this study show that PRP has a positive effect on bone generation. HA with a combination of BMMSCs and PRP can enhance bone regeneration. In addition, the growth factor capacity of PRP may affect the differentiation of BMMSCs and promote bone formation.

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