Synergistic effect of muramyl dipeptide with heat shock protein 70 from Mycobacterium tuberculosis on immune activation

Tae Hyoun Kim, Jong Hwan Park, Yeong Min Park, Seung Wook Ryu, SungJae Shin, Jae Hak Park, Dong Jae Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Heat shock protein 70 from Mycobacterium tuberculosis (Mtb Hsp70) has been known to modulate immune response including dendritic cell activation. Muramyl dipeptide (MDP) is an immunoreactive derivative of peptidoglycan from all Gram-negative and Gram-positive bacteria and recognized to be responsible for function of Freund's complete adjuvant. In this study, we evaluated effect of MDP on in vitro activation of bone marrow derived dendritic cells (BMDCs) and in vivo production of cytokines and chemokines induced by Mtb Hsp70.MDP treatment with Mtb Hsp70 dramatically increased production of IL-6, IL-12p40 and TNF-α in BMDCs compared with Mtb Hsp70 alone whereas these effects were abolished in Nod2-deficient BMDCs. Phosphorylation of IκB-α and ERK and impairment of phagocytosis, which is an indicator of DC maturation were enhanced by MDP co-treatment with Mtb hsp70 in BMDCs. In addition, ability of Mtb Hsp70-stimulated BMDCs to induce IFN-γ productions of T cells was increased by MDP co-treatment. Finally, intraperitoneal injection of MDP with Mtb Hsp70 dramatically increased production of IL-6, CXCL-1 and CCL2 in serum compared with Mtb hsp70 injection.Our study showed the synergistic effects of MDP with Mtb Hsp70 on DCs and in vivo immune activation. The use of MDP with Mtb Hsp70 to induce immune activation may provide an effective strategy for vaccination to treat cancer and protect against pathogens.

Original languageEnglish
Pages (from-to)26-31
Number of pages6
JournalImmunobiology
Volume220
Issue number1
DOIs
Publication statusPublished - 2016 Jan 1

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Acetylmuramyl-Alanyl-Isoglutamine
HSP70 Heat-Shock Proteins
Mycobacterium tuberculosis
Dendritic Cells
Bone Marrow
Interleukin-6
Interleukin-12 Subunit p40
Freund's Adjuvant
Peptidoglycan
Gram-Positive Bacteria
Intraperitoneal Injections
Phagocytosis
Chemokines
Vaccination
Therapeutics
Phosphorylation
Cytokines
T-Lymphocytes
Injections

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Hematology

Cite this

Kim, Tae Hyoun ; Park, Jong Hwan ; Park, Yeong Min ; Ryu, Seung Wook ; Shin, SungJae ; Park, Jae Hak ; Kim, Dong Jae. / Synergistic effect of muramyl dipeptide with heat shock protein 70 from Mycobacterium tuberculosis on immune activation. In: Immunobiology. 2016 ; Vol. 220, No. 1. pp. 26-31.
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Synergistic effect of muramyl dipeptide with heat shock protein 70 from Mycobacterium tuberculosis on immune activation. / Kim, Tae Hyoun; Park, Jong Hwan; Park, Yeong Min; Ryu, Seung Wook; Shin, SungJae; Park, Jae Hak; Kim, Dong Jae.

In: Immunobiology, Vol. 220, No. 1, 01.01.2016, p. 26-31.

Research output: Contribution to journalArticle

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