This study was performed to evaluate the inactivation efficiency or synergy of combined ozone and UV processes (combined ozone/UV process) or sequential processes (ozone-UV, UV-ozone) compared with individual unit processes and to investigate the specific roles of ozone, UV and the hydroxyl radical, which is formed as an intermediate in the combined ozone/UV process. The Bacillus subtilis spore, which has often been used as a surrogate microorganism for Cryptosporidium parvum oocysts, was used as a target microorganism. Compared to individual unit processes with ozone or UV, the inactivation of B. subtilis spores by the combined ozone/UV process was enhanced under identical conditions. To investigate the specific roles of ozone and UV in the combined ozone/UV process, sequential ozone-UV and UV-ozone processes were tested for degrees of inactivation. Additionally, the experiment was performed in the presence and absence of tert-butyl alcohol, which acted as a hydroxyl radical scavenger to assess the role of inactivation by the hydroxyl radical in the combined ozone/UV process. Among the five candidate processes, the greatest synergistic effect was observed in the combined ozone/UV process. From the comparison of five candidate processes, the hydroxyl radical and ozone were each determined to significantly enhance the overall inactivation efficiency in the combined ozone/UV process.
Bibliographical noteFunding Information:
This work was supported by Grant No. (R01-2006-000-10250-0) from the basic research program of the Korean Science & Engineering Foundation and the Brain Korea 21 Program of the Ministry of Education.
All Science Journal Classification (ASJC) codes
- Ecological Modelling
- Water Science and Technology
- Waste Management and Disposal