Synthesis and biological characterization of indolicidin analogues

Yong Beom Lim, Jae Chul Pyun, Jong Sang Park

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Indolicidin has been known to have a broad spectrum of antimicrobial activities against Gram negative and positive bacteria. Its eight analogues were chemically synthesized. The analogue design was based on the analysis of sequence to elucidate the role of some residues in the antibacterial mechanism of indolicidin. Bactericidal activities were assayed against Escherichia coli and Proteus vulgaris, and the membrane perturbing abilities of the peptides were assayed using a dye containing liposome. Among the eight analogues, [Gly4,Gly6]-Indo, [Ile6,Ile8]-Indo, [Lys12]-Indo and [Thr2,Tyr9]-Indo showed enhanced antibacterial activities. These results suggest that proline and cationic residues are important in the bactericidal activity of indolicidin. We tried to describe the antimicrobial mechanism of indolicidin with these results.

Original languageEnglish
Pages (from-to)229-233
Number of pages5
JournalJournal of Biochemistry and Molecular Biology
Volume30
Issue number3
Publication statusPublished - 1997 May 31

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Proteus vulgaris
Gram-Positive Bacteria
Gram-Negative Bacteria
Proline
Liposomes
Escherichia coli
Sequence Analysis
Bacteria
Coloring Agents
Membranes
Peptides
indolicidin

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

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Synthesis and biological characterization of indolicidin analogues. / Lim, Yong Beom; Pyun, Jae Chul; Park, Jong Sang.

In: Journal of Biochemistry and Molecular Biology, Vol. 30, No. 3, 31.05.1997, p. 229-233.

Research output: Contribution to journalArticle

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