Synthesis and Characterization of Functional Nanofilm-Coated Live Immune Cells

Jangsun Hwang, Daheui Choi, Moonhyun Choi, Youngmin Seo, Jaewoo Son, Jinkee Hong, Jonghoon Choi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Layer-by-layer (LbL) assembly techniques have been extensively studied in cell biology because of their simplicity of preparation and versatility. The applications of the LbL platform technology using polysaccharides, silicon, and graphene have been investigated. However, the applications of the above-mentioned technology using living cells remain to be fully understood. This study demonstrates a living cell-based LbL platform using various types of living cells. In addition, it confirms that the surplus charge on the outer surface of the coated cells can be used to bind the target protein. We develop a living cell-based LbL platform technology by stacking layers of hyaluronic acid (HA) and poly-l-lysine (PLL). The HA/PLL stacking results in three bilayers with a thickness of 4 ± 1 nm on the cell surface. Furthermore, the multilayer nanofilms on the cells are completely degraded after 3 days of the application of the LbL method. We also evaluate and visualize three bilayers of the nanofilm on adherent (AML-12 cells)-, nonadherent (trypsin-treated AML-12 cells)-, and circulation type [peripheral blood mononuclear cells (PBMCs)] cells by analyzing the zeta potential, cell viability, and imaging via scanning electron microscopy and confocal microscopy. Finally, we study the cytotoxicity of the nanofilm and characteristic functions of the immune cells after the nanofilm coating. The multilayer nanofilms are not acutely cytotoxic and did not inhibit the immune response of the PBMCs against stimulant. We conclude that a two bilayer nanofilm would be ideal for further study in any cell type. The living cell-based LbL platform is expected to be useful for a variety of applications in cell biology.

Original languageEnglish
Pages (from-to)17685-17692
Number of pages8
JournalACS Applied Materials and Interfaces
Volume10
Issue number21
DOIs
Publication statusPublished - 2018 May 30

Fingerprint

Cells
Cytology
Hyaluronic acid
Hyaluronic Acid
Lysine
Multilayers
Blood
Graphite
Confocal microscopy
Silicon
Zeta potential
Cytotoxicity
Polysaccharides
Trypsin
Graphene
Proteins
Imaging techniques
Coatings
Scanning electron microscopy

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Hwang, Jangsun ; Choi, Daheui ; Choi, Moonhyun ; Seo, Youngmin ; Son, Jaewoo ; Hong, Jinkee ; Choi, Jonghoon. / Synthesis and Characterization of Functional Nanofilm-Coated Live Immune Cells. In: ACS Applied Materials and Interfaces. 2018 ; Vol. 10, No. 21. pp. 17685-17692.
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abstract = "Layer-by-layer (LbL) assembly techniques have been extensively studied in cell biology because of their simplicity of preparation and versatility. The applications of the LbL platform technology using polysaccharides, silicon, and graphene have been investigated. However, the applications of the above-mentioned technology using living cells remain to be fully understood. This study demonstrates a living cell-based LbL platform using various types of living cells. In addition, it confirms that the surplus charge on the outer surface of the coated cells can be used to bind the target protein. We develop a living cell-based LbL platform technology by stacking layers of hyaluronic acid (HA) and poly-l-lysine (PLL). The HA/PLL stacking results in three bilayers with a thickness of 4 ± 1 nm on the cell surface. Furthermore, the multilayer nanofilms on the cells are completely degraded after 3 days of the application of the LbL method. We also evaluate and visualize three bilayers of the nanofilm on adherent (AML-12 cells)-, nonadherent (trypsin-treated AML-12 cells)-, and circulation type [peripheral blood mononuclear cells (PBMCs)] cells by analyzing the zeta potential, cell viability, and imaging via scanning electron microscopy and confocal microscopy. Finally, we study the cytotoxicity of the nanofilm and characteristic functions of the immune cells after the nanofilm coating. The multilayer nanofilms are not acutely cytotoxic and did not inhibit the immune response of the PBMCs against stimulant. We conclude that a two bilayer nanofilm would be ideal for further study in any cell type. The living cell-based LbL platform is expected to be useful for a variety of applications in cell biology.",
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Synthesis and Characterization of Functional Nanofilm-Coated Live Immune Cells. / Hwang, Jangsun; Choi, Daheui; Choi, Moonhyun; Seo, Youngmin; Son, Jaewoo; Hong, Jinkee; Choi, Jonghoon.

In: ACS Applied Materials and Interfaces, Vol. 10, No. 21, 30.05.2018, p. 17685-17692.

Research output: Contribution to journalArticle

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AU - Hwang, Jangsun

AU - Choi, Daheui

AU - Choi, Moonhyun

AU - Seo, Youngmin

AU - Son, Jaewoo

AU - Hong, Jinkee

AU - Choi, Jonghoon

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