Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers

Kevin J. Landmark, Stassi DiMaggio, Jesse Ward, Christopher Kelly, Stefan Vogt, Seungpyo Hong, Alina Kotlyar, Andrzej Myc, Thommey P. Thomas, James E. Penner-Hahn, James R. Baker, Mark M. Banaszak Holl, Bradford G. Orr

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Organic-coated superparamagnetic iron oxide nanoparticles (OC-SPIONs) were synthesized and characterized by transmission electron microscopy and X-ray photoelectron spectroscopy. OC-SPIONs were transferred from organic media into water using poly(amidoamine) dendrimers modified with 6-TAMRA fluorescent dye and folic acid molecules. The saturation magnetization of the resulting dendrimer-coated SPIONs (DC-SPIONs) was determined, using a superconducting quantum interference device, to be 60 emu/g Fe versus 90 emu/g Fe for bulk magnetite. Selective targeting of the DC-SPIONs to KB cancer cells in vitro was demonstrated and quantified using two distinct and complementary imaging modalities: UV-visible and X-ray fluorescence; confocal microscopy confirmed internalization. The results were consistent between the uptake distribution quantified by flow cytometry using 6-TAMRA UV-visible fluorescence intensity and the cellular iron content determined using X-ray fluorescence microscopy.

Original languageEnglish
Pages (from-to)773-783
Number of pages11
JournalACS Nano
Volume2
Issue number4
DOIs
Publication statusPublished - 2008 Apr 1

Fingerprint

folic acid
Dendrimers
dendrimers
Iron oxides
Folic Acid
iron oxides
Nanoparticles
fluorescence
nanoparticles
Acids
Testing
synthesis
Fluorescence
Ferrosoferric Oxide
microscopy
X rays
x rays
cytometry
Flow cytometry
Confocal microscopy

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Landmark, Kevin J. ; DiMaggio, Stassi ; Ward, Jesse ; Kelly, Christopher ; Vogt, Stefan ; Hong, Seungpyo ; Kotlyar, Alina ; Myc, Andrzej ; Thomas, Thommey P. ; Penner-Hahn, James E. ; Baker, James R. ; Banaszak Holl, Mark M. ; Orr, Bradford G. / Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers. In: ACS Nano. 2008 ; Vol. 2, No. 4. pp. 773-783.
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abstract = "Organic-coated superparamagnetic iron oxide nanoparticles (OC-SPIONs) were synthesized and characterized by transmission electron microscopy and X-ray photoelectron spectroscopy. OC-SPIONs were transferred from organic media into water using poly(amidoamine) dendrimers modified with 6-TAMRA fluorescent dye and folic acid molecules. The saturation magnetization of the resulting dendrimer-coated SPIONs (DC-SPIONs) was determined, using a superconducting quantum interference device, to be 60 emu/g Fe versus 90 emu/g Fe for bulk magnetite. Selective targeting of the DC-SPIONs to KB cancer cells in vitro was demonstrated and quantified using two distinct and complementary imaging modalities: UV-visible and X-ray fluorescence; confocal microscopy confirmed internalization. The results were consistent between the uptake distribution quantified by flow cytometry using 6-TAMRA UV-visible fluorescence intensity and the cellular iron content determined using X-ray fluorescence microscopy.",
author = "Landmark, {Kevin J.} and Stassi DiMaggio and Jesse Ward and Christopher Kelly and Stefan Vogt and Seungpyo Hong and Alina Kotlyar and Andrzej Myc and Thomas, {Thommey P.} and Penner-Hahn, {James E.} and Baker, {James R.} and {Banaszak Holl}, {Mark M.} and Orr, {Bradford G.}",
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Landmark, KJ, DiMaggio, S, Ward, J, Kelly, C, Vogt, S, Hong, S, Kotlyar, A, Myc, A, Thomas, TP, Penner-Hahn, JE, Baker, JR, Banaszak Holl, MM & Orr, BG 2008, 'Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers', ACS Nano, vol. 2, no. 4, pp. 773-783. https://doi.org/10.1021/nn800034w

Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers. / Landmark, Kevin J.; DiMaggio, Stassi; Ward, Jesse; Kelly, Christopher; Vogt, Stefan; Hong, Seungpyo; Kotlyar, Alina; Myc, Andrzej; Thomas, Thommey P.; Penner-Hahn, James E.; Baker, James R.; Banaszak Holl, Mark M.; Orr, Bradford G.

In: ACS Nano, Vol. 2, No. 4, 01.04.2008, p. 773-783.

Research output: Contribution to journalArticle

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AU - Kotlyar, Alina

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AU - Thomas, Thommey P.

AU - Penner-Hahn, James E.

AU - Baker, James R.

AU - Banaszak Holl, Mark M.

AU - Orr, Bradford G.

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N2 - Organic-coated superparamagnetic iron oxide nanoparticles (OC-SPIONs) were synthesized and characterized by transmission electron microscopy and X-ray photoelectron spectroscopy. OC-SPIONs were transferred from organic media into water using poly(amidoamine) dendrimers modified with 6-TAMRA fluorescent dye and folic acid molecules. The saturation magnetization of the resulting dendrimer-coated SPIONs (DC-SPIONs) was determined, using a superconducting quantum interference device, to be 60 emu/g Fe versus 90 emu/g Fe for bulk magnetite. Selective targeting of the DC-SPIONs to KB cancer cells in vitro was demonstrated and quantified using two distinct and complementary imaging modalities: UV-visible and X-ray fluorescence; confocal microscopy confirmed internalization. The results were consistent between the uptake distribution quantified by flow cytometry using 6-TAMRA UV-visible fluorescence intensity and the cellular iron content determined using X-ray fluorescence microscopy.

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