Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers

Kevin J. Landmark, Stassi DiMaggio, Jesse Ward, Christopher Kelly, Stefan Vogt, Seungpyo Hong, Alina Kotlyar, Andrzej Myc, Thommey P. Thomas, James E. Penner-Hahn, James R. Baker, Mark M. Banaszak Holl, Bradford G. Orr

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134 Citations (Scopus)

Abstract

Organic-coated superparamagnetic iron oxide nanoparticles (OC-SPIONs) were synthesized and characterized by transmission electron microscopy and X-ray photoelectron spectroscopy. OC-SPIONs were transferred from organic media into water using poly(amidoamine) dendrimers modified with 6-TAMRA fluorescent dye and folic acid molecules. The saturation magnetization of the resulting dendrimer-coated SPIONs (DC-SPIONs) was determined, using a superconducting quantum interference device, to be 60 emu/g Fe versus 90 emu/g Fe for bulk magnetite. Selective targeting of the DC-SPIONs to KB cancer cells in vitro was demonstrated and quantified using two distinct and complementary imaging modalities: UV-visible and X-ray fluorescence; confocal microscopy confirmed internalization. The results were consistent between the uptake distribution quantified by flow cytometry using 6-TAMRA UV-visible fluorescence intensity and the cellular iron content determined using X-ray fluorescence microscopy.

Original languageEnglish
Pages (from-to)773-783
Number of pages11
JournalACS Nano
Volume2
Issue number4
DOIs
Publication statusPublished - 2008 Apr 1

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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    Landmark, K. J., DiMaggio, S., Ward, J., Kelly, C., Vogt, S., Hong, S., Kotlyar, A., Myc, A., Thomas, T. P., Penner-Hahn, J. E., Baker, J. R., Banaszak Holl, M. M., & Orr, B. G. (2008). Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers. ACS Nano, 2(4), 773-783. https://doi.org/10.1021/nn800034w