Synthesis of a highly metal-selective rhodamine-based probe and its use for the in vivomonitoring of mercury

Young Keun Yang; Sung Kyun Ko; Injae Shin; Jinsung Tae

doi:10.1038/nprot.2007.246

Synthesis of a highly metal-selective rhodamine-based probe and its use for the in vivomonitoring of mercury

Young Keun Yang, Sung Kyun Ko, Injae Shin, Jinsung Tae

Department of Chemistry

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89 Citations (Scopus)

Abstract

This protocol describes detailed procedures for the preparation of a rhodamine-based mercury probe and for its applications to the detection of mercury in cells and vertebrate organisms. The mercury probe 1, which is prepared in two steps from rhodamine 6G, responds rapidly to Hg2+ in aqueous solutions with a 1:1 stoichiometry. Owing to the fact that the probe reacts with Hg2+ in an irreversible manner, it has advantages over other reversible mercury probes in in vivo assays with respect to both sensitivity and selectivity. In addition, fluorescent imaging assays of Hg2+ in live cells and zebrafish by using this mercury probe are detailed in this protocol. The approximate time frame for the preparation of the probe is 24 h and for its use in imaging assays is 1.5 h.

Original language	English
Pages (from-to)	1740-1745
Number of pages	6
Journal	Nature Protocols
Volume	2
Issue number	7
DOIs	https://doi.org/10.1038/nprot.2007.246
Publication status	Published - 2007 Jun 28

Bibliographical note

Funding Information:
ACKNOWLEDGMENTS This work was supported by the SRC programs (R11-2000-070 and R11-2003-019) and the NRL program of MOST/KOSEF. Y.-K.Y. and S.-K.K. thank the BK21 program (KRF).

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

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title = "Synthesis of a highly metal-selective rhodamine-based probe and its use for the in vivomonitoring of mercury",

abstract = "This protocol describes detailed procedures for the preparation of a rhodamine-based mercury probe and for its applications to the detection of mercury in cells and vertebrate organisms. The mercury probe 1, which is prepared in two steps from rhodamine 6G, responds rapidly to Hg2+ in aqueous solutions with a 1:1 stoichiometry. Owing to the fact that the probe reacts with Hg2+ in an irreversible manner, it has advantages over other reversible mercury probes in in vivo assays with respect to both sensitivity and selectivity. In addition, fluorescent imaging assays of Hg2+ in live cells and zebrafish by using this mercury probe are detailed in this protocol. The approximate time frame for the preparation of the probe is 24 h and for its use in imaging assays is 1.5 h.",

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note = "Funding Information: ACKNOWLEDGMENTS This work was supported by the SRC programs (R11-2000-070 and R11-2003-019) and the NRL program of MOST/KOSEF. Y.-K.Y. and S.-K.K. thank the BK21 program (KRF).",

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AU - Ko, Sung Kyun

AU - Shin, Injae

AU - Tae, Jinsung

N1 - Funding Information: ACKNOWLEDGMENTS This work was supported by the SRC programs (R11-2000-070 and R11-2003-019) and the NRL program of MOST/KOSEF. Y.-K.Y. and S.-K.K. thank the BK21 program (KRF).

PY - 2007/6/28

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N2 - This protocol describes detailed procedures for the preparation of a rhodamine-based mercury probe and for its applications to the detection of mercury in cells and vertebrate organisms. The mercury probe 1, which is prepared in two steps from rhodamine 6G, responds rapidly to Hg2+ in aqueous solutions with a 1:1 stoichiometry. Owing to the fact that the probe reacts with Hg2+ in an irreversible manner, it has advantages over other reversible mercury probes in in vivo assays with respect to both sensitivity and selectivity. In addition, fluorescent imaging assays of Hg2+ in live cells and zebrafish by using this mercury probe are detailed in this protocol. The approximate time frame for the preparation of the probe is 24 h and for its use in imaging assays is 1.5 h.

AB - This protocol describes detailed procedures for the preparation of a rhodamine-based mercury probe and for its applications to the detection of mercury in cells and vertebrate organisms. The mercury probe 1, which is prepared in two steps from rhodamine 6G, responds rapidly to Hg2+ in aqueous solutions with a 1:1 stoichiometry. Owing to the fact that the probe reacts with Hg2+ in an irreversible manner, it has advantages over other reversible mercury probes in in vivo assays with respect to both sensitivity and selectivity. In addition, fluorescent imaging assays of Hg2+ in live cells and zebrafish by using this mercury probe are detailed in this protocol. The approximate time frame for the preparation of the probe is 24 h and for its use in imaging assays is 1.5 h.

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Synthesis of a highly metal-selective rhodamine-based probe and its use for the in vivomonitoring of mercury

Abstract

Bibliographical note

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