Abstract
This protocol describes detailed procedures for the preparation of a rhodamine-based mercury probe and for its applications to the detection of mercury in cells and vertebrate organisms. The mercury probe 1, which is prepared in two steps from rhodamine 6G, responds rapidly to Hg2+ in aqueous solutions with a 1:1 stoichiometry. Owing to the fact that the probe reacts with Hg2+ in an irreversible manner, it has advantages over other reversible mercury probes in in vivo assays with respect to both sensitivity and selectivity. In addition, fluorescent imaging assays of Hg2+ in live cells and zebrafish by using this mercury probe are detailed in this protocol. The approximate time frame for the preparation of the probe is 24 h and for its use in imaging assays is 1.5 h.
| Original language | English |
|---|---|
| Pages (from-to) | 1740-1745 |
| Number of pages | 6 |
| Journal | Nature Protocols |
| Volume | 2 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2007 Jun 28 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
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Synthesis of a highly metal-selective rhodamine-based probe and its use for the in vivomonitoring of mercury. / Yang, Young Keun; Ko, Sung Kyun; Shin, Injae; Tae, Jinsung.
In: Nature Protocols, Vol. 2, No. 7, 28.06.2007, p. 1740-1745.Research output: Contribution to journal › Article
TY - JOUR
T1 - Synthesis of a highly metal-selective rhodamine-based probe and its use for the in vivomonitoring of mercury
AU - Yang, Young Keun
AU - Ko, Sung Kyun
AU - Shin, Injae
AU - Tae, Jinsung
PY - 2007/6/28
Y1 - 2007/6/28
N2 - This protocol describes detailed procedures for the preparation of a rhodamine-based mercury probe and for its applications to the detection of mercury in cells and vertebrate organisms. The mercury probe 1, which is prepared in two steps from rhodamine 6G, responds rapidly to Hg2+ in aqueous solutions with a 1:1 stoichiometry. Owing to the fact that the probe reacts with Hg2+ in an irreversible manner, it has advantages over other reversible mercury probes in in vivo assays with respect to both sensitivity and selectivity. In addition, fluorescent imaging assays of Hg2+ in live cells and zebrafish by using this mercury probe are detailed in this protocol. The approximate time frame for the preparation of the probe is 24 h and for its use in imaging assays is 1.5 h.
AB - This protocol describes detailed procedures for the preparation of a rhodamine-based mercury probe and for its applications to the detection of mercury in cells and vertebrate organisms. The mercury probe 1, which is prepared in two steps from rhodamine 6G, responds rapidly to Hg2+ in aqueous solutions with a 1:1 stoichiometry. Owing to the fact that the probe reacts with Hg2+ in an irreversible manner, it has advantages over other reversible mercury probes in in vivo assays with respect to both sensitivity and selectivity. In addition, fluorescent imaging assays of Hg2+ in live cells and zebrafish by using this mercury probe are detailed in this protocol. The approximate time frame for the preparation of the probe is 24 h and for its use in imaging assays is 1.5 h.
UR - http://www.scopus.com/inward/record.url?scp=34548552598&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548552598&partnerID=8YFLogxK
U2 - 10.1038/nprot.2007.246
DO - 10.1038/nprot.2007.246
M3 - Article
C2 - 17641639
AN - SCOPUS:34548552598
VL - 2
SP - 1740
EP - 1745
JO - Nature Protocols
JF - Nature Protocols
SN - 1754-2189
IS - 7
ER -