Synthesis of a unique polysialoganglioside by polysialyltransferase from embryos of sea urchin Lytechinus pictus

Size determination of polysialic acid chain synthesized onto GD3 in vitro

Research output: Contribution to journalArticle

Abstract

It has been reported that polysialyltransferase in sea urchin embryos polysialylates 38 kDa endogenous acceptor proteins and disialyllactose moieties of mammalian ganglioside GD3, and its maximum activity was observed at gastrula stage using an excess of GD3 as an exogenous acceptor. The objective of the present investigation is to determine how many sialic acid residues are transferred onto the disialyllactose moiety of GD3 by the polysialyltransferase when it is used as an exogenous acceptor. Ceramide glycanase which can cleave the linkage between ceramide and sugar moiety was treated to obtain hydrophilic polysialyllactose from polysialylated GD3. Surprisingly, size analysis of these chains using non-SDS polyacrylamide gel electrophoresis showed that up to 60 sialic acid residues were transferred to the disialyllactose moiety of GD3 by polysialyltransferase from the sea urchin embryos.

Original languageEnglish
Pages (from-to)989-995
Number of pages7
JournalBiochemistry and Molecular Biology International
Volume44
Issue number5
Publication statusPublished - 1998 Jan 1

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Lytechinus
Size determination
Sea Urchins
Embryonic Structures
N-Acetylneuraminic Acid
Gastrula
Ceramides
Electrophoresis
Sugars
Polyacrylamide Gel Electrophoresis
polysialic acid
disialyllactose
trisialoganglioside GT1
In Vitro Techniques
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

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abstract = "It has been reported that polysialyltransferase in sea urchin embryos polysialylates 38 kDa endogenous acceptor proteins and disialyllactose moieties of mammalian ganglioside GD3, and its maximum activity was observed at gastrula stage using an excess of GD3 as an exogenous acceptor. The objective of the present investigation is to determine how many sialic acid residues are transferred onto the disialyllactose moiety of GD3 by the polysialyltransferase when it is used as an exogenous acceptor. Ceramide glycanase which can cleave the linkage between ceramide and sugar moiety was treated to obtain hydrophilic polysialyllactose from polysialylated GD3. Surprisingly, size analysis of these chains using non-SDS polyacrylamide gel electrophoresis showed that up to 60 sialic acid residues were transferred to the disialyllactose moiety of GD3 by polysialyltransferase from the sea urchin embryos.",
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