Synthesis of aminated polysorbate 80 for polyplex-mediated gene transfection

Eun Kyung Lim, Jaemoon Yang, Jin Suck Suh, Yong Min Huh, Seungjoo Haam

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To develop novel gene delivery carriers, aminated polysorbate 80 (P80-NH2) was synthesized with strong positively charged properties through the introduction of three amine groups. The resulting P80-NH2 and DNA polyplex exhibited superb condensation abilities due to the high densities of positively charged amines groups. Size and surface charge of polyplex were shown to be well suited for cellular internalization. In addition, the P80-NH2/DNA polyplex demonstrated acceptable transfection efficiency in HeLa cells and was nontoxic relative to the conventional 25-kDa polyethyleneimine system.

Original languageEnglish
Pages (from-to)1528-1533
Number of pages6
JournalBiotechnology Progress
Volume26
Issue number6
DOIs
Publication statusPublished - 2010 Nov 1

Fingerprint

Polysorbates
Amines
Transfection
Polyethyleneimine
DNA
HeLa Cells
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology

Cite this

@article{6ad513ed1c544beca38b32c3191aa45b,
title = "Synthesis of aminated polysorbate 80 for polyplex-mediated gene transfection",
abstract = "To develop novel gene delivery carriers, aminated polysorbate 80 (P80-NH2) was synthesized with strong positively charged properties through the introduction of three amine groups. The resulting P80-NH2 and DNA polyplex exhibited superb condensation abilities due to the high densities of positively charged amines groups. Size and surface charge of polyplex were shown to be well suited for cellular internalization. In addition, the P80-NH2/DNA polyplex demonstrated acceptable transfection efficiency in HeLa cells and was nontoxic relative to the conventional 25-kDa polyethyleneimine system.",
author = "Lim, {Eun Kyung} and Jaemoon Yang and Suh, {Jin Suck} and Huh, {Yong Min} and Seungjoo Haam",
year = "2010",
month = "11",
day = "1",
doi = "10.1002/btpr.475",
language = "English",
volume = "26",
pages = "1528--1533",
journal = "Biotechnology Progress",
issn = "8756-7938",
publisher = "John Wiley and Sons Ltd",
number = "6",

}

Synthesis of aminated polysorbate 80 for polyplex-mediated gene transfection. / Lim, Eun Kyung; Yang, Jaemoon; Suh, Jin Suck; Huh, Yong Min; Haam, Seungjoo.

In: Biotechnology Progress, Vol. 26, No. 6, 01.11.2010, p. 1528-1533.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Synthesis of aminated polysorbate 80 for polyplex-mediated gene transfection

AU - Lim, Eun Kyung

AU - Yang, Jaemoon

AU - Suh, Jin Suck

AU - Huh, Yong Min

AU - Haam, Seungjoo

PY - 2010/11/1

Y1 - 2010/11/1

N2 - To develop novel gene delivery carriers, aminated polysorbate 80 (P80-NH2) was synthesized with strong positively charged properties through the introduction of three amine groups. The resulting P80-NH2 and DNA polyplex exhibited superb condensation abilities due to the high densities of positively charged amines groups. Size and surface charge of polyplex were shown to be well suited for cellular internalization. In addition, the P80-NH2/DNA polyplex demonstrated acceptable transfection efficiency in HeLa cells and was nontoxic relative to the conventional 25-kDa polyethyleneimine system.

AB - To develop novel gene delivery carriers, aminated polysorbate 80 (P80-NH2) was synthesized with strong positively charged properties through the introduction of three amine groups. The resulting P80-NH2 and DNA polyplex exhibited superb condensation abilities due to the high densities of positively charged amines groups. Size and surface charge of polyplex were shown to be well suited for cellular internalization. In addition, the P80-NH2/DNA polyplex demonstrated acceptable transfection efficiency in HeLa cells and was nontoxic relative to the conventional 25-kDa polyethyleneimine system.

UR - http://www.scopus.com/inward/record.url?scp=78650180424&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650180424&partnerID=8YFLogxK

U2 - 10.1002/btpr.475

DO - 10.1002/btpr.475

M3 - Article

C2 - 20662085

AN - SCOPUS:78650180424

VL - 26

SP - 1528

EP - 1533

JO - Biotechnology Progress

JF - Biotechnology Progress

SN - 8756-7938

IS - 6

ER -