Synthesis of aminated polysorbate 80 for polyplex-mediated gene transfection

Eun Kyung Lim, Jaemoon Yang, Jin Suck Suh, Yong Min Huh, Seungjoo Haam

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To develop novel gene delivery carriers, aminated polysorbate 80 (P80-NH2) was synthesized with strong positively charged properties through the introduction of three amine groups. The resulting P80-NH2 and DNA polyplex exhibited superb condensation abilities due to the high densities of positively charged amines groups. Size and surface charge of polyplex were shown to be well suited for cellular internalization. In addition, the P80-NH2/DNA polyplex demonstrated acceptable transfection efficiency in HeLa cells and was nontoxic relative to the conventional 25-kDa polyethyleneimine system.

Original languageEnglish
Pages (from-to)1528-1533
Number of pages6
JournalBiotechnology Progress
Volume26
Issue number6
DOIs
Publication statusPublished - 2010 Nov 1

All Science Journal Classification (ASJC) codes

  • Biotechnology

Fingerprint Dive into the research topics of 'Synthesis of aminated polysorbate 80 for polyplex-mediated gene transfection'. Together they form a unique fingerprint.

  • Cite this