Synthesis of an Hsp70 inhibitor and its assessment of lysosomal membrane permeabilization

Sang Hyun Park; Sookil Park; Injae Shin

doi:10.1016/j.xpro.2021.100349

Synthesis of an Hsp70 inhibitor and its assessment of lysosomal membrane permeabilization

Sang Hyun Park, Sookil Park, Injae Shin

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

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Abstract

Hsp70 inhibitors have great potential as chemical probes and anticancer agents. Thus, it is important to elucidate their modes of action on cancer cell death. This protocol describes a step-by-step process for the synthesis of apoptozole as an inhibitor of Hsp70, analysis of internalization of apoptozole into lysosomes, and assessment of lysosomal membrane permeabilization induced by apoptozole. The current protocol can be used for detailed mechanistic studies of Hsp70 inhibitors and further substances targeting lysosomal proteins on cancer cell death. For complete information on the use and execution of this protocol, please refer to Park et al. (2018).

Original language	English
Article number	100349
Journal	STAR Protocols
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2021.100349
Publication status	Published - 2021 Mar 19

Bibliographical note

Funding Information:
This study was supported financially by the National Research Foundation of Korea (grant no. 2020R1A2C3003462 ).

Publisher Copyright:
© 2021 The Author(s)

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
Neuroscience(all)

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10.1016/j.xpro.2021.100349

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abstract = "Hsp70 inhibitors have great potential as chemical probes and anticancer agents. Thus, it is important to elucidate their modes of action on cancer cell death. This protocol describes a step-by-step process for the synthesis of apoptozole as an inhibitor of Hsp70, analysis of internalization of apoptozole into lysosomes, and assessment of lysosomal membrane permeabilization induced by apoptozole. The current protocol can be used for detailed mechanistic studies of Hsp70 inhibitors and further substances targeting lysosomal proteins on cancer cell death. For complete information on the use and execution of this protocol, please refer to Park et al. (2018).",

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AU - Shin, Injae

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AB - Hsp70 inhibitors have great potential as chemical probes and anticancer agents. Thus, it is important to elucidate their modes of action on cancer cell death. This protocol describes a step-by-step process for the synthesis of apoptozole as an inhibitor of Hsp70, analysis of internalization of apoptozole into lysosomes, and assessment of lysosomal membrane permeabilization induced by apoptozole. The current protocol can be used for detailed mechanistic studies of Hsp70 inhibitors and further substances targeting lysosomal proteins on cancer cell death. For complete information on the use and execution of this protocol, please refer to Park et al. (2018).

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Synthesis of an Hsp70 inhibitor and its assessment of lysosomal membrane permeabilization

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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