Synthesis of an Hsp70 inhibitor and its assessment of lysosomal membrane permeabilization

Sang Hyun Park, Sookil Park, Injae Shin

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Hsp70 inhibitors have great potential as chemical probes and anticancer agents. Thus, it is important to elucidate their modes of action on cancer cell death. This protocol describes a step-by-step process for the synthesis of apoptozole as an inhibitor of Hsp70, analysis of internalization of apoptozole into lysosomes, and assessment of lysosomal membrane permeabilization induced by apoptozole. The current protocol can be used for detailed mechanistic studies of Hsp70 inhibitors and further substances targeting lysosomal proteins on cancer cell death. For complete information on the use and execution of this protocol, please refer to Park et al. (2018).

Original languageEnglish
Article number100349
JournalSTAR Protocols
Issue number1
Publication statusPublished - 2021 Mar 19

Bibliographical note

Funding Information:
This study was supported financially by the National Research Foundation of Korea (grant no. 2020R1A2C3003462 ).

Publisher Copyright:
© 2021 The Author(s)

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)


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