Synthesis of Quercetin-3-O-Glucoside from rutin by penicillium decumbens naringinase

Young Su Lee, Ji Young Huh, So Hyun Nam, Doman Kim, Soo Bok Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Enzymatic bioconversion of rutin to quercetin-3-O-glucoside (Q-3-G) by Penicillium decumbens naringinase was increased with reaction pH increased approximately to pH 6.0. It resulted in greater than 92% production of Q-3-G due to the removal of the terminal rhamnose at the controlled pH 6.0. The enzymatic bioconversion of rutin to Q-3-G was repetitively performed, yielding 84% after 5 batches with little quercetin formation. Interestingly, the water solubility of Q-3-G was enhanced 69- and 328-fold over those of rutin and quercetin, which may make Q-3-G more bioavailable in food. Q-3-G was approximately 6- and 1.4-fold more potent than rutin as an inhibitor of human intestinal maltase and human DL-3-hydroxy-3-methylglutalyl coenzyme A reductase. Q-3-G was less potent (16- and 1.3-fold, respectively) than quercetin as an inhibitor of these enzymes. However, the results suggest that Q-3-G may be confirmed more effective and bioavailable food component than rutin and even quercetin because of its enhanced solubility and inhibitory properties.

Original languageEnglish
Pages (from-to)C411-C415
JournalJournal of Food Science
Volume78
Issue number3
DOIs
Publication statusPublished - 2013 Mar 1

Fingerprint

Penicillium decumbens
naringinase
Rutin
Penicillium
rutin
quercetin
glucosides
synthesis
Quercetin
Solubility
biotransformation
Food
Rhamnose
alpha-Glucosidases
isoquercitrin
Enzyme Inhibitors
Coenzyme A
Oxidoreductases
coenzyme A
rhamnose

All Science Journal Classification (ASJC) codes

  • Food Science

Cite this

Lee, Young Su ; Huh, Ji Young ; Nam, So Hyun ; Kim, Doman ; Lee, Soo Bok. / Synthesis of Quercetin-3-O-Glucoside from rutin by penicillium decumbens naringinase. In: Journal of Food Science. 2013 ; Vol. 78, No. 3. pp. C411-C415.
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Synthesis of Quercetin-3-O-Glucoside from rutin by penicillium decumbens naringinase. / Lee, Young Su; Huh, Ji Young; Nam, So Hyun; Kim, Doman; Lee, Soo Bok.

In: Journal of Food Science, Vol. 78, No. 3, 01.03.2013, p. C411-C415.

Research output: Contribution to journalArticle

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