MicroProteins are small, single-domain proteins that regulate multidomain proteins by sequestering them into novel, often nonproductive, complexes. Several microProteins have been identified in plants and animals, most of which negatively regulate transcription factors. MicroProtein candidates that potentially target a wide range of different protein classes were recently identified in a computational approach. Here, we classified all Arabidopsis (Arabidopsis thaliana) microProteins and developed a synthetic microProtein approach to target specific protein classes, such as hydrolases, receptors, and lyases, in a proof-of-concept approach. Our findings reveal that microProteins can be used to influence different physiological processes, which makes them useful tools for posttranslational regulation in plants and potentially also in animals.
Bibliographical noteFunding Information:
1This work was supported by the European Research Council (GA336295) and the Copenhagen Plant Science Centre. 2 These authors contributed equally to the article. 3 Current address: Zentrum für Quantitative Biologie (QBiC), University of Tübingen, Auf der Morgenstelle 10, 72076 Tübingen, Germany. 4 Address correspondence to firstname.lastname@example.org. The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Stephan Wenkel (email@example.com.\*506793218dk\*506793218). U.D., V.R., and S.W. conceived the original screening and research plan; S.W. supervised the experiments; U.D., V.R., D.S., K.K.B., and S.C. performed the experiments; U.D. designed the experiments and analyzed the data; U.D. and S.W. wrote the article with contributions from all authors. [OPEN] Articles can be viewed without a subscription. www.plantphysiol.org/cgi/doi/10.1104/pp.17.01743
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All Science Journal Classification (ASJC) codes
- Plant Science