Synthetic ratiometric fluorescent probes for detection of ions

Sang Hyun Park, Nahyun Kwon, Jee Hyeon Lee, Juyoung Yoon, Injae Shin

Research output: Contribution to journalReview articlepeer-review

412 Citations (Scopus)


Metal cations and anions are essential for versatile physiological processes. Dysregulation of specific ion levels in living organisms is known to have an adverse effect on normal biological events. Owing to the pathophysiological significance of ions, sensitive and selective methods to detect these species in biological systems are in high demand. Because they can be used in methods for precise and quantitative analysis of ions, organic dye-based ratiometric fluorescent probes have been extensively explored in recent years. In this review, recent advances (2015-2019) made in the development and biological applications of synthetic ratiometric fluorescent probes are described. Particular emphasis is given to organic dye-based ratiometric fluorescent probes that are designed to detect biologically important and relevant ions in cells and living organisms. Also, the fundamental principles associated with the design of ratiometric fluorescent probes and perspectives about how to expand their biological applications are discussed.

Original languageEnglish
Pages (from-to)143-179
Number of pages37
JournalChemical Society reviews
Issue number1
Publication statusPublished - 2020 Jan 7

Bibliographical note

Funding Information:
This work was supported financially by the National Research Foundation of Korea (grant no. 2019R1H1A3037339 to I. S. and 2012R1A3A2048814 to J. Y.).

Publisher Copyright:
© The Royal Society of Chemistry 2020.

All Science Journal Classification (ASJC) codes

  • Chemistry(all)


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