Systematic analysis of cDNA microarray-based CGH

Chan Hee Park, Ha Jin Jeong, Yeon Ho Choi, Sang Cheol Kim, Hei Chul Jeong, Kyu Hyun Park, Gui Yeon Lee, Tae Soo Kim, Sang Wha Yang, Sung Whan Ahn, Yang Seok Kim, SunYoung Rha, Hyuncheol Chung

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16 Citations (Scopus)

Abstract

cDNA microarray-based CGH (Microarray-CGH) is a useful technique for detecting genomic aberrations with a high resolution. However, the criteria for determining a genomic alteration have not been determined. We evaluated the genome-wide measurement of copy number of each gene in normal gastric and placenta tissues with both sex-matched, direct and sex-mismatched, indirect designs using 17K cDNA microarray. The results revealed the range of genomic copy number of normal tissues to be ±0.3 of the log 2 ratio (gain >0.3, loss <-0.3) in the autosomal genes with direct and indirect designs. The copy number at a gene level from the X chromosomal genes using the direct and indirect sex-mismatched designs was ±0.68 of the log2 ratio (amplification >0.68, deletion <-0.68). In summary, the suggested method can be used as a guideline for analysis of genomic aberration using a Microarray-CGH in both direct and indirect designs.

Original languageEnglish
Pages (from-to)261-267
Number of pages7
JournalInternational Journal of Molecular Medicine
Volume17
Issue number2
Publication statusPublished - 2006 Feb 1

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Oligonucleotide Array Sequence Analysis
Gene Dosage
Placenta
Stomach
Genome
Guidelines

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Park, C. H., Jeong, H. J., Choi, Y. H., Kim, S. C., Jeong, H. C., Park, K. H., ... Chung, H. (2006). Systematic analysis of cDNA microarray-based CGH. International Journal of Molecular Medicine, 17(2), 261-267.
Park, Chan Hee ; Jeong, Ha Jin ; Choi, Yeon Ho ; Kim, Sang Cheol ; Jeong, Hei Chul ; Park, Kyu Hyun ; Lee, Gui Yeon ; Kim, Tae Soo ; Yang, Sang Wha ; Ahn, Sung Whan ; Kim, Yang Seok ; Rha, SunYoung ; Chung, Hyuncheol. / Systematic analysis of cDNA microarray-based CGH. In: International Journal of Molecular Medicine. 2006 ; Vol. 17, No. 2. pp. 261-267.
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Park, CH, Jeong, HJ, Choi, YH, Kim, SC, Jeong, HC, Park, KH, Lee, GY, Kim, TS, Yang, SW, Ahn, SW, Kim, YS, Rha, S & Chung, H 2006, 'Systematic analysis of cDNA microarray-based CGH', International Journal of Molecular Medicine, vol. 17, no. 2, pp. 261-267.

Systematic analysis of cDNA microarray-based CGH. / Park, Chan Hee; Jeong, Ha Jin; Choi, Yeon Ho; Kim, Sang Cheol; Jeong, Hei Chul; Park, Kyu Hyun; Lee, Gui Yeon; Kim, Tae Soo; Yang, Sang Wha; Ahn, Sung Whan; Kim, Yang Seok; Rha, SunYoung; Chung, Hyuncheol.

In: International Journal of Molecular Medicine, Vol. 17, No. 2, 01.02.2006, p. 261-267.

Research output: Contribution to journalArticle

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AU - Lee, Gui Yeon

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AU - Yang, Sang Wha

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Park CH, Jeong HJ, Choi YH, Kim SC, Jeong HC, Park KH et al. Systematic analysis of cDNA microarray-based CGH. International Journal of Molecular Medicine. 2006 Feb 1;17(2):261-267.