Systematic analysis of cDNA microarray-based CGH

Chan Hee Park, Ha Jin Jeong, Yeon Ho Choi, Sang Cheol Kim, Hei Chul Jeong, Kyu Hyun Park, Gui Yeon Lee, Tae Soo Kim, Sang Wha Yang, Sung Whan Ahn, Yang Seok Kim, Sun Young Rha, Hyun Cheol Chung

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16 Citations (Scopus)

Abstract

cDNA microarray-based CGH (Microarray-CGH) is a useful technique for detecting genomic aberrations with a high resolution. However, the criteria for determining a genomic alteration have not been determined. We evaluated the genome-wide measurement of copy number of each gene in normal gastric and placenta tissues with both sex-matched, direct and sex-mismatched, indirect designs using 17K cDNA microarray. The results revealed the range of genomic copy number of normal tissues to be ±0.3 of the log2 ratio (gain >0.3, loss <-0.3) in the autosomal genes with direct and indirect designs. The copy number at a gene level from the X chromosomal genes using the direct and indirect sex-mismatched designs was ±0.68 of the log2 ratio (amplification >0.68, deletion <-0.68). In summary, the suggested method can be used as a guideline for analysis of genomic aberration using a Microarray-CGH in both direct and indirect designs.

Original languageEnglish
Pages (from-to)261-267
Number of pages7
JournalInternational journal of molecular medicine
Volume17
Issue number2
Publication statusPublished - 2006 Feb 1

All Science Journal Classification (ASJC) codes

  • Genetics

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    Park, C. H., Jeong, H. J., Choi, Y. H., Kim, S. C., Jeong, H. C., Park, K. H., Lee, G. Y., Kim, T. S., Yang, S. W., Ahn, S. W., Kim, Y. S., Rha, S. Y., & Chung, H. C. (2006). Systematic analysis of cDNA microarray-based CGH. International journal of molecular medicine, 17(2), 261-267.