Systematic error in gas chromatography-mass spectrometry-based quantification of hydrolyzed urinary steroids

Ju Yeon Moon, Young Wan Ha, Myeong Hee Moon, Bong Chul Chung, Man Ho Choi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Gas chromatography-mass spectrometry-based metabolite profiling can lead to an understanding of various disease mechanisms as well as to identifying new diagnostic biomarkers by comparing the metabolites related in quantification. However, the unexpected transformation of urinary steroids during enzymatic hydrolysis with Helix pomatia could result in an underestimation or overestimation of their concentrations. A comparison of β-glucurondase extracted from Escherichia coli revealed 18 conversions of 84 steroids tested as an unexpected transformation under hydrolysis with β-glucuronidase/ arylsulfatase extracted from Helix pomatia. In addition to the conversion of 3β-hydroxy-5-ene steroids into 3-oxo-4-ene steroids, which has been reported, the transformation of 3β-hydroxy-5α-reduced and 3β-hydroxy-5β-reduced steroids to 3-oxo-5α-reduced and 3-oxo-5β-reduced steroids, respectively, was newly observed. The formation of by-products was in proportion to the concentration of substrates becoming saturated against the enzyme. The substances belonging to these three steroid groups were undetectable at low concentrations, whereas the corresponding by-products were overestimated. These results indicate that the systematic error in the quantification of urinary steroids hydrolyzed with Helix pomatia can lead to a misreading of the clinical implications. All these hydrolysis procedures are suitable for study purposes, and the information can help prevent false evaluations of urinary steroids in clinical studies.

Original languageEnglish
Pages (from-to)388-397
Number of pages10
JournalCancer Epidemiology Biomarkers and Prevention
Volume19
Issue number2
DOIs
Publication statusPublished - 2010 Feb 1

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Oncology

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