Systemic transplantation of human adipose-derived stem cells stimulates bone repair by promoting osteoblast and osteoclast function

Kyunghee Lee, Hyunsoo Kim, Jin Man Kim, Jae Ryong Kim, Keuk Jun Kim, Yong Jin Kim, Se Il Park, Jae Ho Jeong, Young mi Moon, Hyun Sook Lim, Dong Won Bae, Joseph Kwon, Chang Yong Ko, Hansung Kim, Hong In Shin, Daewon Jeong

Research output: Contribution to journalArticle

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Abstract

Systemic transplantation of adipose-derived stem cells (ASCs) is emerging as a novel therapeutic option for functional recovery of diverse damaged tissues. This study investigated the effects of systemic transplantation of human ASCs (hASCs) on bone repair. We found that hASCs secrete various bone cell-activating factors, including hepatocyte growth factor and extracellular matrix proteins. Systemic transplantation of hASCs into ovariectomized mice induced an increased number of both osteoblasts and osteoclasts in bone tissue and thereby prevented bone loss. We also observed that conditioned medium from hASCs is capable of stimulating proliferation and differentiation of osteoblasts via Smad/extracellular signal-regulated kinase (ERK)/JNK (c-jun NH 2-terminal kinase) activation as well as survival and differentiation of osteoclasts via ERK/JNK/p38 activation in vitro. Overall, our findings suggest that paracrine factors secreted from hASCs improve bone repair and that hASCs can be a valuable tool for use in osteoporosis therapy.

Original languageEnglish
Pages (from-to)2082-2094
Number of pages13
JournalJournal of Cellular and Molecular Medicine
Volume15
Issue number10
DOIs
Publication statusPublished - 2011 Oct 1

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Osteoclasts
Osteoblasts
Stem Cells
Transplantation
Bone and Bones
Extracellular Signal-Regulated MAP Kinases
Hepatocyte Growth Factor
Extracellular Matrix Proteins
Conditioned Culture Medium
Osteoporosis
Phosphotransferases
Survival
Therapeutics

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Cell Biology

Cite this

Lee, Kyunghee ; Kim, Hyunsoo ; Kim, Jin Man ; Kim, Jae Ryong ; Kim, Keuk Jun ; Kim, Yong Jin ; Park, Se Il ; Jeong, Jae Ho ; Moon, Young mi ; Lim, Hyun Sook ; Bae, Dong Won ; Kwon, Joseph ; Ko, Chang Yong ; Kim, Hansung ; Shin, Hong In ; Jeong, Daewon. / Systemic transplantation of human adipose-derived stem cells stimulates bone repair by promoting osteoblast and osteoclast function. In: Journal of Cellular and Molecular Medicine. 2011 ; Vol. 15, No. 10. pp. 2082-2094.
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abstract = "Systemic transplantation of adipose-derived stem cells (ASCs) is emerging as a novel therapeutic option for functional recovery of diverse damaged tissues. This study investigated the effects of systemic transplantation of human ASCs (hASCs) on bone repair. We found that hASCs secrete various bone cell-activating factors, including hepatocyte growth factor and extracellular matrix proteins. Systemic transplantation of hASCs into ovariectomized mice induced an increased number of both osteoblasts and osteoclasts in bone tissue and thereby prevented bone loss. We also observed that conditioned medium from hASCs is capable of stimulating proliferation and differentiation of osteoblasts via Smad/extracellular signal-regulated kinase (ERK)/JNK (c-jun NH 2-terminal kinase) activation as well as survival and differentiation of osteoclasts via ERK/JNK/p38 activation in vitro. Overall, our findings suggest that paracrine factors secreted from hASCs improve bone repair and that hASCs can be a valuable tool for use in osteoporosis therapy.",
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Lee, K, Kim, H, Kim, JM, Kim, JR, Kim, KJ, Kim, YJ, Park, SI, Jeong, JH, Moon, YM, Lim, HS, Bae, DW, Kwon, J, Ko, CY, Kim, H, Shin, HI & Jeong, D 2011, 'Systemic transplantation of human adipose-derived stem cells stimulates bone repair by promoting osteoblast and osteoclast function', Journal of Cellular and Molecular Medicine, vol. 15, no. 10, pp. 2082-2094. https://doi.org/10.1111/j.1582-4934.2010.01230.x

Systemic transplantation of human adipose-derived stem cells stimulates bone repair by promoting osteoblast and osteoclast function. / Lee, Kyunghee; Kim, Hyunsoo; Kim, Jin Man; Kim, Jae Ryong; Kim, Keuk Jun; Kim, Yong Jin; Park, Se Il; Jeong, Jae Ho; Moon, Young mi; Lim, Hyun Sook; Bae, Dong Won; Kwon, Joseph; Ko, Chang Yong; Kim, Hansung; Shin, Hong In; Jeong, Daewon.

In: Journal of Cellular and Molecular Medicine, Vol. 15, No. 10, 01.10.2011, p. 2082-2094.

Research output: Contribution to journalArticle

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AU - Kim, Keuk Jun

AU - Kim, Yong Jin

AU - Park, Se Il

AU - Jeong, Jae Ho

AU - Moon, Young mi

AU - Lim, Hyun Sook

AU - Bae, Dong Won

AU - Kwon, Joseph

AU - Ko, Chang Yong

AU - Kim, Hansung

AU - Shin, Hong In

AU - Jeong, Daewon

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