Target deconvolution of bioactive small molecules: The heart of chemical biology and drug discovery

Hye Jin Jung, Ho Jeong Kwon

Research output: Contribution to journalReview article

24 Citations (Scopus)


Identification of the target proteins of bioactive small molecules isolated from phenotypic screens plays an important role in chemical biology and drug discovery. However, discovering the targets of small molecules is often the most challenging and time-consuming step for chemical biology researchers. To overcome the bottlenecks in target identification, many new approaches based on genomics, proteomics, and bioinformatics technologies have been developed. Here, we provide an overview of the current major methodologies for target deconvolution of bioactive small molecules. To obtain an integrated view of the mechanisms of action of small molecules, we propose a systematic approach that involves the combination of multi-omics-based target identification and validation and preclinical target validation.

Original languageEnglish
Pages (from-to)1627-1641
Number of pages15
JournalArchives of pharmacal research
Issue number9
Publication statusPublished - 2015 Sep 22


All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Drug Discovery
  • Organic Chemistry

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