Targeted optical injection of gold nanoparticles into single mammalian cells

Craig McDougall, David J. Stevenson, Christian T.A. Brown, Frank Gunn-Moore, Kishan Dholakia

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We present an all optical technique for the targeted delivery of single 100 nm diameter gold nanoparticles into a specified region of the interior of an individual mammalian cell through a combination of optical tweezing and optical injection. The internalisation of the nanoparticle is verified by confocal laser scanning microscopy and confocal laser scanning reflectance microscopy. This represents the first time that nano sized particles have been tweezed and optically injected into mammalian cells using only light, and provides a novel methodology for internalising nanosphere based biosensors within specific intracellular regions of a mammalian cell.

Original languageEnglish
Pages (from-to)736-743
Number of pages8
JournalJournal of Biophotonics
Volume2
Issue number12
DOIs
Publication statusPublished - 2009 Dec 1

Fingerprint

Gold
Nanoparticles
Cells
injection
gold
Confocal Microscopy
nanoparticles
Injections
Microscopic examination
Nanospheres
microscopy
Scanning
scanning
Lasers
Biosensing Techniques
bioinstrumentation
Biosensors
lasers
delivery
methodology

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

McDougall, Craig ; Stevenson, David J. ; Brown, Christian T.A. ; Gunn-Moore, Frank ; Dholakia, Kishan. / Targeted optical injection of gold nanoparticles into single mammalian cells. In: Journal of Biophotonics. 2009 ; Vol. 2, No. 12. pp. 736-743.
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Targeted optical injection of gold nanoparticles into single mammalian cells. / McDougall, Craig; Stevenson, David J.; Brown, Christian T.A.; Gunn-Moore, Frank; Dholakia, Kishan.

In: Journal of Biophotonics, Vol. 2, No. 12, 01.12.2009, p. 736-743.

Research output: Contribution to journalArticle

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