Targeting phospholipase D1 attenuates intestinal tumorigenesis by controlling β-catenin signaling in cancer-initiating cells

Dong Woo Kang, Chi Yeol Choi, Yong Hee Cho, Huasong Tian, Gilbert Di Paolo, Kang Yell Choi, Do Sik Min

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Expression of the Wnt target gene phospholipase D1 (PLD1) is up-regulated in various carcinomas, including colorectal cancer (CRC). However, the mechanistic significance of its elevated expression in intestinal tumorigenesis remains unknown. In this study, we show that genetic and pharmacological targeting of PLD1 disrupts spontaneous and colitis-associated intestinal tumorigenesis in ApcMin/+ and azoxymethane/dextran sodium sulfate mice models. Intestinal epithelial cell-specific PLD1 overexpression in ApcMin/+ mice accelerated tumorigenesis with increased proliferation and nuclear β-catenin levels compared with ApcMin/+ mice. Moreover, PLD1 inactivation suppressed the self-renewal capacity of colon cancer-initiating cells (CC-ICs) by decreasing expression of β-catenin via E2F1-induced microRNA (miR)-4496 up-regulation. Ultimately, low expression of PLD1 coupled with a low level of CC-IC markers was predictive of a good prognosis in CRC patients, suggesting in vivo relevance. Collectively, our data reveal that PLD1 has a crucial role in intestinal tumorigenesis via its modulation of the E2F1-miR-4496-β-catenin signaling pathway. Modulation of PLD1 expression and activity represents a promising therapeutic strategy for the treatment of intestinal tumorigenesis.

Original languageEnglish
Pages (from-to)1219-1237
Number of pages19
JournalJournal of Experimental Medicine
Issue number8
Publication statusPublished - 2015 Jul 27

Bibliographical note

Funding Information:
This study was supported by a National Research Foundation of Korea grant funded by the Korean government (MEST; 2012002009), a Translational Research Center for Protein Function Control grant (National Science Foundation 2009-0083522), and a National R&D Program for Cancer Control grant from the Ministry for Health, Welfare, and Family Affairs (Republic of Korea; 0920050). The authors declare no competing financial interests.

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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