Tautomycetin inhibits growth of colorectal cancer cells through p21cip/WAF1 induction via the extracellular signal-regulated kinase pathway

Joon Hee Lee, Jung Soo Lee, Sung Eun Kim, Byoung San Moon, Yong Chul Kim, Seung Kyou Lee, Sang Kyou Lee, Kang-Yell Choi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Tautomycetin is an antifungal antibiotic retaining potent immunosuppressive function. We have identified the roles of tautomycetin on cellular proliferation and transformation of colorectal cancer cells. The proliferation and anchorage-independent growth of HCT-15, HT-29, and DLD-1 colorectal cancer cells were efficiently inhibited without induction of apoptosis by 150 nmol tautomycetin. These growth inhibitory effects were dependent on p21Cip/WAF induction via the extracellular signal - regulated kinase pathway, and the tautomycetin effects were abolished in HCT-116 colon cells and eight other types of cells that did not induce P21Cip/WAF by 150 nmol tautomycetin. The crucial role of p21Cip/WAF1 in the extracellular signal - regulated kinase pathway-dependent antiproliferative responses by tautomycetin was confirmed by using p21Cip/WAF1 gene-deleted HCT-116 cells. The growth inhibitory effect of tautomycetin was acquired by regulation of Raf-1 activity through inhibition of protein phosphatase type 1 and protein phosphatase type 2A with high preference toward protein phosphatase type 1. Tautomycetin could be a potential drug for colorectal cancer.

Original languageEnglish
Pages (from-to)3222-3231
Number of pages10
JournalMolecular Cancer Therapeutics
Volume5
Issue number12
DOIs
Publication statusPublished - 2006 Dec 1

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Extracellular Signal-Regulated MAP Kinases
Colorectal Neoplasms
Growth
HCT116 Cells
Protein Phosphatase 1
Protein Phosphatase 2
tautomycetin
Antifungal Agents
Immunosuppressive Agents
Colon
Cell Proliferation
Apoptosis
Pharmaceutical Preparations
Genes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Lee, Joon Hee ; Lee, Jung Soo ; Kim, Sung Eun ; Moon, Byoung San ; Kim, Yong Chul ; Lee, Seung Kyou ; Lee, Sang Kyou ; Choi, Kang-Yell. / Tautomycetin inhibits growth of colorectal cancer cells through p21cip/WAF1 induction via the extracellular signal-regulated kinase pathway. In: Molecular Cancer Therapeutics. 2006 ; Vol. 5, No. 12. pp. 3222-3231.
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Tautomycetin inhibits growth of colorectal cancer cells through p21cip/WAF1 induction via the extracellular signal-regulated kinase pathway. / Lee, Joon Hee; Lee, Jung Soo; Kim, Sung Eun; Moon, Byoung San; Kim, Yong Chul; Lee, Seung Kyou; Lee, Sang Kyou; Choi, Kang-Yell.

In: Molecular Cancer Therapeutics, Vol. 5, No. 12, 01.12.2006, p. 3222-3231.

Research output: Contribution to journalArticle

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