Telomerase- and angiogenesis-related gene responses to irradiation in human umbilical vein endothelial cells

Hyun Chang, Sun Young Rha, Hei Cheul Jeung, Kyu Hyun Park, Tae Soo Kim, Yong Bae Kim, Hyun Cheol Chung

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, we investigated the effects of ionizing radiation (IR) on human umbilical vein endothelial cells (HUVECs) in the context of senescence. HUVECs at passage number (PN)1, PN2 and PN3 were exposed to irradiation (2 Gy). The growth rate of the HUVECS was measured by proliferation assay and senescence-associated ß-galactosidase assay was used to measure the number of senescent cells. Telomerase activity and the expression of telomerase- and angiogenesis-related genes were measured by telomerase assay and real-time PCR, respectively. The number of senescent cells was significantly increased in the irradiated HUVECs at all PNs. Compared to the controls, telomerase activity, the expression of human telomerase reverse transcriptase (hTERT) and c-Myc in the irradiated HUVECs were downregulated during serial passage. The downregulation of vascular endothelial growth factor (VEGF) was observed in the irradiated HUVECs as the PN increased. The data presented in this study may aid in the understanding of the mechanisms behind IR-induced EC senescence and telomerase- and angiogenesis-related gene response.

Original languageEnglish
Pages (from-to)1202-1208
Number of pages7
JournalInternational journal of molecular medicine
Volume31
Issue number5
DOIs
Publication statusPublished - 2013 May 1

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Telomerase
Human Umbilical Vein Endothelial Cells
Genes
Ionizing Radiation
Down-Regulation
Cell Count
Galactosidases
Serial Passage
Vascular Endothelial Growth Factor A
Real-Time Polymerase Chain Reaction
Growth

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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abstract = "In this study, we investigated the effects of ionizing radiation (IR) on human umbilical vein endothelial cells (HUVECs) in the context of senescence. HUVECs at passage number (PN)1, PN2 and PN3 were exposed to irradiation (2 Gy). The growth rate of the HUVECS was measured by proliferation assay and senescence-associated {\ss}-galactosidase assay was used to measure the number of senescent cells. Telomerase activity and the expression of telomerase- and angiogenesis-related genes were measured by telomerase assay and real-time PCR, respectively. The number of senescent cells was significantly increased in the irradiated HUVECs at all PNs. Compared to the controls, telomerase activity, the expression of human telomerase reverse transcriptase (hTERT) and c-Myc in the irradiated HUVECs were downregulated during serial passage. The downregulation of vascular endothelial growth factor (VEGF) was observed in the irradiated HUVECs as the PN increased. The data presented in this study may aid in the understanding of the mechanisms behind IR-induced EC senescence and telomerase- and angiogenesis-related gene response.",
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Telomerase- and angiogenesis-related gene responses to irradiation in human umbilical vein endothelial cells. / Chang, Hyun; Rha, Sun Young; Jeung, Hei Cheul; Park, Kyu Hyun; Kim, Tae Soo; Kim, Yong Bae; Chung, Hyun Cheol.

In: International journal of molecular medicine, Vol. 31, No. 5, 01.05.2013, p. 1202-1208.

Research output: Contribution to journalArticle

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