Temporal cDNA microarray analysis of gene expression in human hepatocellular carcinoma upon radiation exposure

Jaemin Jeong, Su Jin Hong, Yeun Jin Ju, Bu Yeo Kim, Myung Jin Park, Tae Hwan Kim, Chul In Park, Kang-Yell Choi, Myung Haeng Cho, Sang Hoon Kim, Hansoo Lee, Kee Ho Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Radiotherapy is not commonly used for the treatment of human hepatocellular carcinoma, due to its poor response rate and poor tolerance of normal liver to ionizing radiation. Recently developed microarray technology makes it possible to verify genes responsive to anticancer therapy of human cancers by simultaneous analysis of gene expression profiles. In the present study, the expression profile of radiation-responsive genes in human hepatocellular carcinoma was evaluated through time-dependent cDNA microarray analysis of expressional variation, following exposure to ionizing radiation. Upon exposure to radiation, more than 13% of genes in both radiation-resistant and -sensitive cells responded to radiation. Time-dependent analysis of radiation-responsive genes revealed that, irrespective of radiation sensitivity, greatly different subsets of genes sequentially participated in cellular response to radiation at their specific activation or deactivation time points. The majority of radiation-responsive genes were differentially but not commonly expressed between radiation-resistant and -sensitive cells. When these differentially regulated genes were classified according to their physiological and functional characteristics and radiation sensitivity, it was prominently obvious that DNA repair-promoting genes were up-regulated in radio-resistant cells and down-regulated or unchanged in radiation-sensitive cells. The present findings indicate that different subsets of genes are sequentially working and DNA repair capacity may control the radiation sensitivity of human hepatocellular carcinoma cells more than any other physiological factor.

Original languageEnglish
Pages (from-to)33-48
Number of pages16
JournalOncology Reports
Volume15
Issue number1
Publication statusPublished - 2006 Jan 1

Fingerprint

Microarray Analysis
Oligonucleotide Array Sequence Analysis
Hepatocellular Carcinoma
Gene Expression
Radiation
Genes
Radiation Tolerance
Ionizing Radiation
DNA Repair
Radiation Exposure
Radio
Transcriptome
Radiotherapy
Technology
Liver
Therapeutics

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Jeong, J., Hong, S. J., Ju, Y. J., Kim, B. Y., Park, M. J., Kim, T. H., ... Lee, K. H. (2006). Temporal cDNA microarray analysis of gene expression in human hepatocellular carcinoma upon radiation exposure. Oncology Reports, 15(1), 33-48.
Jeong, Jaemin ; Hong, Su Jin ; Ju, Yeun Jin ; Kim, Bu Yeo ; Park, Myung Jin ; Kim, Tae Hwan ; Park, Chul In ; Choi, Kang-Yell ; Cho, Myung Haeng ; Kim, Sang Hoon ; Lee, Hansoo ; Lee, Kee Ho. / Temporal cDNA microarray analysis of gene expression in human hepatocellular carcinoma upon radiation exposure. In: Oncology Reports. 2006 ; Vol. 15, No. 1. pp. 33-48.
@article{f6b2ce00f4c947a5a67ee53732db5050,
title = "Temporal cDNA microarray analysis of gene expression in human hepatocellular carcinoma upon radiation exposure",
abstract = "Radiotherapy is not commonly used for the treatment of human hepatocellular carcinoma, due to its poor response rate and poor tolerance of normal liver to ionizing radiation. Recently developed microarray technology makes it possible to verify genes responsive to anticancer therapy of human cancers by simultaneous analysis of gene expression profiles. In the present study, the expression profile of radiation-responsive genes in human hepatocellular carcinoma was evaluated through time-dependent cDNA microarray analysis of expressional variation, following exposure to ionizing radiation. Upon exposure to radiation, more than 13{\%} of genes in both radiation-resistant and -sensitive cells responded to radiation. Time-dependent analysis of radiation-responsive genes revealed that, irrespective of radiation sensitivity, greatly different subsets of genes sequentially participated in cellular response to radiation at their specific activation or deactivation time points. The majority of radiation-responsive genes were differentially but not commonly expressed between radiation-resistant and -sensitive cells. When these differentially regulated genes were classified according to their physiological and functional characteristics and radiation sensitivity, it was prominently obvious that DNA repair-promoting genes were up-regulated in radio-resistant cells and down-regulated or unchanged in radiation-sensitive cells. The present findings indicate that different subsets of genes are sequentially working and DNA repair capacity may control the radiation sensitivity of human hepatocellular carcinoma cells more than any other physiological factor.",
author = "Jaemin Jeong and Hong, {Su Jin} and Ju, {Yeun Jin} and Kim, {Bu Yeo} and Park, {Myung Jin} and Kim, {Tae Hwan} and Park, {Chul In} and Kang-Yell Choi and Cho, {Myung Haeng} and Kim, {Sang Hoon} and Hansoo Lee and Lee, {Kee Ho}",
year = "2006",
month = "1",
day = "1",
language = "English",
volume = "15",
pages = "33--48",
journal = "Oncology Reports",
issn = "1021-335X",
publisher = "Spandidos Publications",
number = "1",

}

Jeong, J, Hong, SJ, Ju, YJ, Kim, BY, Park, MJ, Kim, TH, Park, CI, Choi, K-Y, Cho, MH, Kim, SH, Lee, H & Lee, KH 2006, 'Temporal cDNA microarray analysis of gene expression in human hepatocellular carcinoma upon radiation exposure', Oncology Reports, vol. 15, no. 1, pp. 33-48.

Temporal cDNA microarray analysis of gene expression in human hepatocellular carcinoma upon radiation exposure. / Jeong, Jaemin; Hong, Su Jin; Ju, Yeun Jin; Kim, Bu Yeo; Park, Myung Jin; Kim, Tae Hwan; Park, Chul In; Choi, Kang-Yell; Cho, Myung Haeng; Kim, Sang Hoon; Lee, Hansoo; Lee, Kee Ho.

In: Oncology Reports, Vol. 15, No. 1, 01.01.2006, p. 33-48.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Temporal cDNA microarray analysis of gene expression in human hepatocellular carcinoma upon radiation exposure

AU - Jeong, Jaemin

AU - Hong, Su Jin

AU - Ju, Yeun Jin

AU - Kim, Bu Yeo

AU - Park, Myung Jin

AU - Kim, Tae Hwan

AU - Park, Chul In

AU - Choi, Kang-Yell

AU - Cho, Myung Haeng

AU - Kim, Sang Hoon

AU - Lee, Hansoo

AU - Lee, Kee Ho

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Radiotherapy is not commonly used for the treatment of human hepatocellular carcinoma, due to its poor response rate and poor tolerance of normal liver to ionizing radiation. Recently developed microarray technology makes it possible to verify genes responsive to anticancer therapy of human cancers by simultaneous analysis of gene expression profiles. In the present study, the expression profile of radiation-responsive genes in human hepatocellular carcinoma was evaluated through time-dependent cDNA microarray analysis of expressional variation, following exposure to ionizing radiation. Upon exposure to radiation, more than 13% of genes in both radiation-resistant and -sensitive cells responded to radiation. Time-dependent analysis of radiation-responsive genes revealed that, irrespective of radiation sensitivity, greatly different subsets of genes sequentially participated in cellular response to radiation at their specific activation or deactivation time points. The majority of radiation-responsive genes were differentially but not commonly expressed between radiation-resistant and -sensitive cells. When these differentially regulated genes were classified according to their physiological and functional characteristics and radiation sensitivity, it was prominently obvious that DNA repair-promoting genes were up-regulated in radio-resistant cells and down-regulated or unchanged in radiation-sensitive cells. The present findings indicate that different subsets of genes are sequentially working and DNA repair capacity may control the radiation sensitivity of human hepatocellular carcinoma cells more than any other physiological factor.

AB - Radiotherapy is not commonly used for the treatment of human hepatocellular carcinoma, due to its poor response rate and poor tolerance of normal liver to ionizing radiation. Recently developed microarray technology makes it possible to verify genes responsive to anticancer therapy of human cancers by simultaneous analysis of gene expression profiles. In the present study, the expression profile of radiation-responsive genes in human hepatocellular carcinoma was evaluated through time-dependent cDNA microarray analysis of expressional variation, following exposure to ionizing radiation. Upon exposure to radiation, more than 13% of genes in both radiation-resistant and -sensitive cells responded to radiation. Time-dependent analysis of radiation-responsive genes revealed that, irrespective of radiation sensitivity, greatly different subsets of genes sequentially participated in cellular response to radiation at their specific activation or deactivation time points. The majority of radiation-responsive genes were differentially but not commonly expressed between radiation-resistant and -sensitive cells. When these differentially regulated genes were classified according to their physiological and functional characteristics and radiation sensitivity, it was prominently obvious that DNA repair-promoting genes were up-regulated in radio-resistant cells and down-regulated or unchanged in radiation-sensitive cells. The present findings indicate that different subsets of genes are sequentially working and DNA repair capacity may control the radiation sensitivity of human hepatocellular carcinoma cells more than any other physiological factor.

UR - http://www.scopus.com/inward/record.url?scp=33644873360&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644873360&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 33

EP - 48

JO - Oncology Reports

JF - Oncology Reports

SN - 1021-335X

IS - 1

ER -