Temporospatial tissue interactions regulating the regeneration of the enamel knot in the developing mouse tooth

Sung Won Cho, Jae Young Kim, Jinglei Cai, Jong Min Lee, Eun Jung Kim, Hyun A. Lee, Hitoshi Yamamoto, Hansung Jung

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The enamel knot (EK), which is a transient signaling center in the tooth germ, regulates both the differential growth of the dental epithelium and the tooth shape. In this study, the regeneration of the EK was evaluated. The EK regions were removed from the E14 and E16 dental epithelia, and the remaining epithelia were recombined with their original dental mesenchymes. All these tooth germs could develop into calcified teeth after being transplanted into the kidney capsule for 3 weeks. One primary EK was regenerated earlier, and two or three secondary EKs were regenerated later in culture. When simply recombined without removing the EK, the tooth germ, which had four secondary EKs and four cuspal areas of the dental papilla, generated one primary EK first and subsequent secondary EKs. These results indicate that the patterning of the EK in all tooth germs always starts from a primary EK independent of the direct epithelial or mesenchymal control. This suggests that neither the dental epithelium nor the dental mesenchyme can dictate the pattern or number of the EK formation, but the interaction between the dental epithelium and the dental mesenchyme is essential for the regeneration and patterning of the EKs.

Original languageEnglish
Pages (from-to)158-165
Number of pages8
JournalDifferentiation
Volume75
Issue number2
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

Dental Enamel
Regeneration
Tooth
Tooth Germ
Epithelium
Mesoderm
Dental Papilla
Capsules
Kidney
Growth

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

Cite this

Cho, Sung Won ; Kim, Jae Young ; Cai, Jinglei ; Lee, Jong Min ; Kim, Eun Jung ; Lee, Hyun A. ; Yamamoto, Hitoshi ; Jung, Hansung. / Temporospatial tissue interactions regulating the regeneration of the enamel knot in the developing mouse tooth. In: Differentiation. 2007 ; Vol. 75, No. 2. pp. 158-165.
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Temporospatial tissue interactions regulating the regeneration of the enamel knot in the developing mouse tooth. / Cho, Sung Won; Kim, Jae Young; Cai, Jinglei; Lee, Jong Min; Kim, Eun Jung; Lee, Hyun A.; Yamamoto, Hitoshi; Jung, Hansung.

In: Differentiation, Vol. 75, No. 2, 01.01.2007, p. 158-165.

Research output: Contribution to journalArticle

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