TGF-β activity related to the use of collagen membranes: In vitro bioassays

Layla Panahipour, Zahra Kargarpour, Bernadette Luza, Jung Seok Lee, Reinhard Gruber

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Collagen membranes commonly used in guided bone regeneration are supposed to actively influence tissue regeneration and are not exclusively serving as passive barriers shielding away the soft tissue. The molecular mechanisms by which collagen membranes might affect tissue regeneration might involve the activation of transforming growth factor beta (TGF-β) signaling pathways. Here, we determined the TGF-β activity of supernatants and proteolytic lysates of five commercially available collagen membranes. The expression of TGF-β target genes interleukin 11 (IL11), NADPH oxidase 4 (NOX4), and proteoglycan 4 (PRG4) was evaluated by reverse transcriptase polymerase chain reaction and IL11 immunoassay in gingival fibroblasts. TGF-β signaling activation was further assessed by blocking the TGF-β receptor I kinase, a TGF-β neutralizing antibody, and showing the nuclear localization of phosphorylated Smad3 and total Smad2/3. We could identify two collagen membranes whose supernatants and lysates caused a robust increase of TGF-β receptor I kinase-dependent expression of IL11 in gingival fibroblasts. Moreover, the supernatant of a particular one membrane caused the nuclear localization of phosphorylated Smad3 and Smad2/3 in the fibroblasts. These results strengthen the evidence that some collagen membranes possess an intrinsic TGF-β activity that might actively influence the process of guided bone regeneration.

Original languageEnglish
Article number6636
Pages (from-to)1-10
Number of pages10
JournalInternational journal of molecular sciences
Volume21
Issue number18
DOIs
Publication statusPublished - 2020 Sep 2

Bibliographical note

Funding Information:
Open Access Funding by the Austrian Science Fund (FWF). R.G. received support from FWF (4072-B28).

Funding Information:
Funding: Open Access Funding by the Austrian Science Fund (FWF). R.G. received support from FWF (4072-B28).

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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